再生障碍性贫血大鼠模型骨髓微血管密度表达的实验研究

Bone Marrow Angiogenesis in a Rat Model for Aplastic Anemia

目的研究再生障碍性贫血(再障)大鼠模型骨髓微血管密度的表达。方法将22只Wistar大鼠随机分为两组,造模组12只,对照组10只。造模组给予腹腔注射氟尿嘧啶注射液和灌胃白消安。对照组给等量生理盐水。全部给药结束后1周,采集静脉血进行血常规检测,处死大鼠,肝脏和股骨头切片进行苏木精-伊红染色,用免疫组织化学法检测骨髓微血管的表达,用CD34抗体标记骨髓组织微血管,用磷酸盐缓冲液做空白对照。用图像分析软件Image Pro Plus 6.0对微血管密度的表达做定量分析。结果造模完成后,造模组存活8只,对照组存活10只。造模组大鼠白细胞、红细胞、血红蛋白和血小板计数均较对照组显著减少,肝脏和骨髓苏木素-伊红染色呈现明显的再障病理改变。8例模型组大鼠微血管密度的平均光密度值为0.34±0.04,10例对照组大鼠的平均光密度值为0.45±0.03,模型组大鼠微血管密度的表达显著低于对照组(t=6.2263,P<0.05)。结论再障大鼠骨髓血管生成减少,微血管密度减低。

Objective To investigate the expression of mierovessel density （MVD） in the bone marrow of a rat model for aplastic anemia. Methods Twenty-two Wistar rats were randomly divided into 2 groups, model group with 12 rats and control group with 10 rats. Model group was administered with 5-fu by perito- neal injection and given busulfan extracts by gavage. Control group was given equivalent saline instead. After one week of all the administration. Blood samples were collected fi）r testing,the rats were executed. Liver and femoral sections were bematoxylin-eosin stained. The expression of mierovessel density in the hone marrow was measured immunohistochemically,and the bone marrow microvessels were marked with CD34 antibody. PBS was used to do a blank control. The results were analyzed by hnage Pro Plus 6.0 to quantify the expres- sion of microvessel density. Results After the model was completed, there were 8 rats in the model group and 10 rats in the control group. Compared with the control group,the count of WBC, RBC, HB and PLT in the model group was significantly decreased, and hematoxylin-eosin staining of liver and bone marrow showed significant pathological changes of aplastic anemia. Mean optical density value of microvascular density in the 8 model rats was 0.34 ＋0.04, while the value of the 10 control rats was 0.45 ＋ 0.03, indicating that rats microvessel density in model group was significantly lower than in control group （ t = 6： 2263, P 〈 O. 05 ）. Conclusion Bone marrow angiogenesis in a rat model far aplastic anemia is decreased and MVD is reduced.