374例系统性红斑狼疮患者自身抗体检测及结果分析

Analysis of Autoantibody Profiles of 374 Cases of Systemic Lupus Erythematosus

目的探讨血清自身抗体检测对系统性红斑狼疮(SLE)的诊断价值和临床意义。方法采用间接免疫荧光法(IIFA)检测SLE患者血清中的抗核抗体(ANA);抗ds-DNA抗体的检测采用免疫斑点金渗滤法;采用酶免疫斑点法检测6种特异性核抗原的自身抗体(抗Sm、RNP、SS-A、SS-B、Scl-70和JO-1抗体)。结果①374例SLE患者中,IIFA检测ANA阴性31例,ANA阳性者343例(阳性率91.7%),主要荧光模式为核颗粒型、核颗粒/均质型;②抗Sm、RNP、SS-A、SS-B、Sc1-70、Jo-1、dsDNA的阳性率分别为15.0%、23.8%、50.5%、12.0%、0%、0.3%和43.8%。结论 ANA的联合检测对SLE的诊断和疗效观察具有重要意义。

Objective To explore the diagnostic value and clinical significance of autoantibody test for SLE patients. Methods Serum samples of 374 SLE patients were analyzed by detecting antinuclear antibody （ ANA）, anti-ds-DNA antidody and ENA antibody repertoire（ anti-Sm, anti-RNP, anti-SS-A, anti-SS-B, anti- Sc170 and anti-Jol antibody）with indirect immunofluorescence assay（IIFA） and gold linked immunodotting percolation and enzyme linked immunodotting respectively. Results （~Among the 374 SLE patients, there were 31 cases of ANA negative of IIFA test, and the positive rate of ANA （IIFA）for SLE patients was 91.7% ,and speckled pattern and speckled/homogeneuous pattern were the major immunofluorescence pat- terns ; （~the positive rate of anti-Sm antibody, anti-RNP antibody, anti-SS-A antibody, anti-SS-B antibody, anti-Scl70 antibody,anti-Jol antibody and anti-dsDNA were 15.0% ,23.8% ,50.5%, 12.0% ,0% ,0.3% and 43.8%. Conclusion Co-detection has great clinical significance for diagnosis and evaluation of cura- tive effect of SLE.