甲烷氧化菌群共代谢降解TCE的动力学研究

Degradation Kinetics of Trichloroethylene by Cometabolism of Methanotrophscolony

好氧降解三氯乙烯(trichloroethylene,TCE)是一种经济有效且环境友好的生物修复技术。从矿化垃圾中富集了甲烷氧化菌群,并利用其静息细胞进行了共代谢降解TCE的研究。当TCE初始浓度为45.5 mg/L,菌体浓度C x为1.728 g/L时,降解率可达到79%。该菌群对TCE有较强的耐受性和亲和性,降解TCE的动力学符合Monad模型。拟合结果表明:最大比消耗速率q s,max为1.51×10-4min-1,半饱和常数K S为2.58 mg/L(R2=0.961)。

Aerobicdegradation of trichloroethylene （TCE） is an economical and environment-friendly biological recovery technique.Methylocystis strains were enriched from aged refuse and the degradation of TCE by cometabolism by its resting cells were studied.The degradation rate reached 79％ when the initial concentration of TCE was 45.5 mg/L and cells concentration Cx was 1.728 g/L,indicating that the Methylocystis strains had higher tolerance and affinity potential to TCE.The maximum specific degradation rate （qs,max =1.51 × 10-4min-1） and half-saturation constants（Ks =2.58 mg/L,R2 =0.961） were calculated according to kinetic equation of TCE degradation which fitted to Monad model well.