期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

PCR-DGGE技术在动物胃肠道菌群多样性研究中的应用 被引量：1

下载PDF
导出
摘要 动物胃肠道内定居着种类和数量庞大的微生物群,胃肠道微生物区系对宿主具有重要的生理作用.研究发现,附殖在人和动物消化道内的大量微生物,形成复杂而动态平衡的微生物区系,这些消化道内微生物区系对宿主的健康和营养物质的消化吸收有着重要的作用[1].当动物胃肠道内菌群失去平衡,就会出现菌群失调,使宿主致病.
作者 徐博文 张琦
机构地区 珠海出入境检验检疫局 四川农业大学动物生物技术中心
出处 《中国兽医杂志》 CAS 北大核心 2013年第10期55-57,共3页 Chinese Journal of Veterinary Medicine
关键词 菌群多样性 胃肠道 DGGE技术 动物 PCR- 肠道微生物区系 应用 微生物群
分类号 S852.6 [农业科学—基础兽医学]
  • 相关文献

参考文献13

  • 1Hooper L V,Melissa H W,Anders T,et al.Molecular anal ysis of commensal host microbial relationships in the intestine[J].Science,2001,291(5505):881-884.
  • 2Randazzo C L,Torriani S,Antoon D L,et al.Diversity,dynamics,and activity of bacterial communities during production of an artisanal Sicilian cheese as evaluated by 16S rRNA analysis[J].Appl Environ Microbiol,2002,68(4):1882-1892.
  • 3Fischer S G,Lerman L S.DNA fragments differing by single base-pair substitutions are separated in denaturing gradient gels: correspondence with melting theory[J].Proceedings of the National Academy of Sciences,1983,80(6):1579-1583.
  • 4Muyzer G,De Waal E C,Uitterlinden A G.Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction amplified genes coding for 16S rRNA[J].Appl Environ Microbiol,1993,59(3):695-700.
  • 5Yu Z,Morrison M.Comparisons of different hypervariable regions of rrs genes for use in fingerprinting of microbial communities by PCR-denaturing gradient gel electrophoresis[J].Appl.Environ.Microbiol.,2004,70(8):4800-4806.
  • 6Da Mota F F,Gomes E A,Paiva E,et al.Use of rpoB gene analysis for identification of nitrogen fixing Paenibacillus species as an alternative to the 16S rRNA gene[J].Lett.Appl.Microbiol.,2004,39(1):34-40.
  • 7Suzuki M,Nakagawa Y,Harayama S,et al.Phylogenetic analysis and taxonomic study of marine Cytophaga-like bacteria:proposal for Tenacibaculum gen.nov.with Tenacibaculum maritimum comb.nov.and Tenacibaculum ovolyticum comb.nov.,and description of Tenacibaculum mesophilum sp.nov.and Tenacibaculum amylolyticum sp.nov[J].International journal of systematic and evolutionary microbiology,2001,51(5):1639-1652.
  • 8Simpson J M,Vance J,McCrackenl H,et al.Denaturing gradient gel electrophoresis analysis of 16S ribosomal DNA amplicons to monitor changes in fecal bacterial populations of weaning pigs after introduction of Lactobacillus reuteri strain MM53[J].Appl Environ Microbiol,2000,66(11):4705-4714.
  • 9石鹏君,柏映国,袁铁铮,姚斌,范云六,周志刚,孟昆,伍宁丰,刁其玉.应用rpoB和16S rDNA基因的变性梯度凝胶电泳技术对山羊瘤胃细菌多样性的研究[J].微生物学报,2007,47(2):285-289. 被引量：22
  • 10Satokari R M,Elaine E,Vaughan,et al.Bifidobacterial diversity in human feces detected by genus-specific PCR and denaturing gradient gel electrophoresis[J].Appl Environ Microbiol,2001,67(2):504-513.

二级参考文献16

  • 1姚文,朱伟云,韩正康,Antoon D L Akkermans,Barbara Williams,Seerp Tamminga.应用变性梯度凝胶电泳和16SrDNA序列分析对山羊瘤胃细菌多样性的研究[J].中国农业科学,2004,37(9):1374-1378. 被引量：53
  • 2山田晴宇，生物工学会志，1998年，76卷，2期，58页
  • 3Hespell RB,Akin DE,Dehority BA.Bacteria,fungi and protozoa of the rumen.Chapman and Hall press,1996.
  • 4Zoetendal EG,Collier CT,Koike S,et al.Molecular ecological analysisof the gastrointestinal microbiota:A review.J Nutrit,2004,134:465-472.
  • 5Muyzer G.DGGE/TGGE a method for identifying genes from natural ecosystems.Curr Opin Microbiol,1999,2:317-322.
  • 6Rochelle PA.Environmental Molecular Microbiology:Protocols and Applications.Wymondham(UK):Horizon Scientific Press,2001,177-190.
  • 7Dahllof I,Baillie H,Kjelleberg S.rpoB-Based microbial community analysis avoids limitations inherent in 16S rRNA gene intraspecies heterogeneity.Appl Environ Microbiol,2000,66:3376-3380.
  • 8Da Mota FF,Gomes EA,Paiva E,et al.Use of rpoB gene analysis for identification of nitrogen-fixing Paenibacillus species as an alternative to the 16S rRNA gene.Lett Appl Microbiol,2004,39:34-40.
  • 9Renouf V,Claisse O,Miot-Sertier C,et al.Lactic acid bacteria evolution during winemaking:Use of rpo B gene as a target for PCR-DGGE analysis.Food microbial,2006,23:136-145.
  • 10Yu Z,Morrison M.Improved extraction of PCR-quality community DNA from digesta and fecal samples.BioTechniques,2004,36:808-812.

共引文献25

同被引文献15

引证文献1

二级引证文献3

中国兽医杂志
2013年 第10期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部