凉山州马铃薯栽培品种的遗传多样性分析

Analysis of Genetic Diversity on Liangshan Cultivated Potatoes

利用SSR分子标记技术对栽培的60份马铃薯品种进行了遗传多样性分析，包括凉山州地方品种、自育品种和各地育成品种，筛选出16对有效SSR引物。采用筛选的16对引物在供试材料中共检测到65个位点，其中多态性位点42个，占总扩增位点的64．62％，平均PIC值为0．4717。通过NTSYSpc2．0对所有材料间的遗传距离进行计算，平均遗传距离在2．43～3．96cM之间。使用MEGA4分析软件将供试材料分成3大类，2个凉山州地方品种在聚类图中最先被分离出来。利用PoP．GEN将凉山州地方种和其余材料分成了2类。试验结果表明部分凉山州地方种与试验中其他品种的遗传基础差异较大，是较好的资源材料。该研究为凉山州地方马铃薯品种在今后育种中的应用提供了部分参考依据。

The genetic diversity of 60 cultivated potatoes which contained Liangshan landraces,varieties bred by xichang college,and varieties bred by other places were analyzed by SSR molecular marker technology. We had screened out 16 effective SSR primers and found 65 loci employing those markers in the test materials. There were 42 polymorphic loci which accounted for 64.62%. The average value of PIC was 0. 4717. The genetic distances a- mong all materials were between 2.43 to 3.96 which had been calculated by NTSYSpc 2.0. The test materials had been divided into 3 categories by MEGA4 software and two Liangshan landraces was first separated out in the den- drogram. The Liangshan landraees and other accessions were classified into two groups by PoPGEN software. Those results indicated definite difference between several Liangshan landraces and other materials in the genetic basisand suggested that several Liangshan landraces were useful resources for breeding. This study provided partial references for application of Liangshan landraces in the further potatoes breeding.