黄淮海大豆异黄酮含量分析与特异种质遴选

Genetic Variability Analysis and Elite Germplasm Selection of Isoflavone Content in Soybean from HuangHuaiHai Ecotype Region

以黄淮海生态区181份栽培大豆与32份野生大豆为材料，采用高效液相色谱法测定其子粒异黄酮及组分含量，分析该地区大豆子粒异黄酮含量遗传变异，遴选高异黄酮特异种质，为相关基因克隆表达、RIL群体构建和专用型品种选育提供资源。结果表明，供试栽培大豆异黄酮含量在1462．6—6115．5μg／g，平均为3558．2μg／g，最大差异可达4．2倍；供试野生大豆异黄酮含量在3896．1—7440．4o-g／g，平均为5182．4μg／g，最大差异可达1．9倍。可见，黄淮海生态区大豆资源异黄酮及组分含量存在较大遗传变异，且野生大豆异黄酮平均含量显著高于栽培大豆。从供试资源中遴选出异黄酮含量超过6000μg／g特异种质4份（超过7000μg／g种质1份）。

Abstract： To analyze the genetic variability and select elite germplasm with high content of total isoflavone and its components in soybean seed, 213 accessions, including 181 cultivated and 32 wild soybean originating from HuangHuaiHai ecological region ,were tested by high performance liquid chromatography （HPLC） technique. The results showed that a large genetic variation in total isoflavone and its components existed in both cultivated and wild soybeans, and the average content of total isoflavone in wild soybean seed was much higher than the cultivated seed. The range of total isoflavone in cultivated soybean was 1462.6-6115.5μg/g （4.2-fold variation） , with an average of 3558.2 μg/g,while the total isoflavone in wild soybean was 3896. 1-7440. 4 μg/g （ 1.9-fold variation） ,with an average of 5182.4 μg/g. From the accessions,four elite germplasm with high isoflavone content （ 〉 6000μg/g） were screened out,of which one elite accession content was above 7000μg/g,and could be used for molecular clo- ning of related genes, construction of RIL population, and new variety breeding with high isoflavone content in fur- ther study.