近红外光谱无损测定大豆种子生活力方法研究

Non-destructive Determination of Soybean Seed Viability by Near-Infrared Spectroscopy

快速准确无损测定种子生活力是种质资源安全保存研究中的一项重要内容。采用傅立叶变换近红外漫反射光谱技术，以保存不同年限的大豆品种资源的种子为样品，利用偏最小二乘法（PLS）建立其生活力的无损测定校正模型，并对不同光谱预处理方法和不同建模波段范围对模型的预测性能进行对比分析。结果表明：利用4000—10000／cm全波段建立的模型预测性能较高。经Savitzky-Golay二阶导数和标准化预处理后，生活力的PLS模型预测性能最好，校正集的决定系数为O．957，验证集决定系数为0．902，校正均方根误差（RMSEC）和验证均方根误差（RMSEV）分别为2．190和2．684。模型预测误差接近标准发芽方法测定误差，说明该方法准确性较高，能够满足种质资源快速、非破坏性生活力检测的要求，为今后快速无损测定种子生活力提供了方法。

A fast,accurate and non-destructive assay of seed viability is a critical research subject for the preservation of germplasm. In this study,a rapid and non-destructive determination assay of seed viability was established by employing fourier transform near-infrared( FT-NIR) spectroscopy. A calibrations model using partial least squares(PLS) was developed for the relationship between FT-NTR wave band and soybean seed viability,where the seeds were processed by different preservation times. Simultaneously,the prediction performance comparisons of several models established from different spectral-pretreatment methods and different wave band were conducted. Results showed that the model with 4000-10000/cm of full-band range demonstrated a higher prediction accuracy,where a Savitzky-Golay second derivative and standardized pretreatment was conducted. The determination coefficient of calibration set and prediction set was 0. 937 and 0. 902,respectively,which was close to that of the standard germination method. RMSEC and RMSEV were 2. 190 and 2. 684,respectively. It indicated that this method had high accuracy,and could be considered as a rapid non-destructive determination protocol of seed viability,which was essential technique for germplasm preservation practice.