中药多成分体系谱动学数学模型的实验验证研究

Validation of chromatogram pharmacokinetic model,a pharmacokinetics for multiple components in Chinese materia medica with fingerprint chromatography

目的:对已建立以指纹图谱表达的中药多成分体系的药物动力学,亦谱动学的总量统计矩数学模型进行实验验证。方法:以补阳还五汤中黄芪甲苷、芍药苷与苦杏仁苷3成分为模型药物,采用反相高效液相色谱法测定,分别用成分叠加拟合、总量统计矩、线性谱动学、梯形谱动学四计算法求算出3成分的谱动学总量统计矩参数。结果:其中线性谱动学计算的总量统计矩参数分别为VUCT为(118.8±14.56)mg·min·mL-1;MRT T为(168.6±6.564)min;VRTT为(89 670±2 089)min2;ClT为(0.4556±0.03505)mL·min-1·kg-1;VT为(76.90±5.238)mL/kg;t0.95T为(622.2±16.89)min;λ0.95T为(106.1±0.6339)min。结论:3成分总量的半衰期为(116.8±5.503)min,3药在0-622.2min内代谢95%的浓度。四法参数无显著性差异。所建立的中药谱动学数学模型及参数体能表征中药多成分体系药物动力学行为。

Objective： To experimentally study on the pharmacokinetics for the multiple components in the Chinese materia medica （CMM） with fingerprint chromatography, as total quantum statistic moment model （TQSMM） in Chromatogram pharmacokinetics. Methods： The RP-HPLC was used with a mobile phase of acetonitrile-water. The parameters of pharmacokinetics for single components were dealt with DAS and the TQSM parameters calculated by Excel software written as equations. The parameter ingredient fitting, total quantum moment, linear Chromatogram pharmacokinetics, trapezoid chromatugram pharmacokinetics. Results： The total quantum parameters had shown VUCr （118.8±14.56）mg.min.mL-l; MRTr （168.6±6.564）min; VRTr （89 670±2 089）min2; Clr （0.4556±0.03505） mL.min-l.kg^-1; Vr （76.90±5.238）mL/kg; t0.95r （622.2±16.89） rain; 2o.95r （106.1±0.6339）min. Conclusion： It is suggested that the half time for three ingredients was （116.8±5.503） min with 95% of ingredient metabolic period as 0-622.2rain; Analyses are carried out with no significant difference by four calculation ways such as overlapped among the linear chromatogram pharmacokinetics, the chromatogram pharmacokinetic model and parameter system can be utilized to study on multiple component pharmacokinetic behavior.