羚羊角及其混伪品脂类成分气相色谱-质谱分析和聚类分析

GC/MSD and cluster analysis of characteristic lipids in Saigae Tataricae Cornu and its adulterants

目的:阐明及鉴别羚羊角及其混伪品中脂类化学成分。方法:溶剂法提取脂类成分,经甲酯化,采用气相色谱-质谱-计算机联用技术分离鉴定,归一化法计算相对百分含量,并进行聚类分析。结果:从5种牛科动物角中共鉴定出13种脂类成分,其中从羚羊角中鉴定出9种脂肪酸类成分;5种牛科动物角采用系统聚类法可分为3类。结论:羚羊角主要脂肪酸类成分为棕榈酸、硬脂酸、油酸,可利用此三指标之比鉴别羚羊角。

Objective： To clarify the characteristic lipid constituents in Saigae Tataricae Cornu and its adulterants. Methods： The lipids were extracted by solvent method, then esterified and identified by capillary gas chromatography-mass spectra-computer technique. Results： 13 lipid components were identified from five kinds of Bovidae horns, and 9 kinds of fatty acids were identified from the Saigae Tataricae Cornu; five kinds of Bovidae horns could be divided into three families by using the system clustering method. Conclusion： Palmitic acid, oleic acid and Stearic acid were the characteristic lipids in Saigae Tataricae Cornu, which could be used as the differential index.