甘蔗热带种Badila蔗糖合成酶基因的克隆及表达分析

Cloning and Sequence Analysis of a Chewing Cane Sucrose Synthase Gene(SoSuS1)and Investigation of Expression Profiling

植物中蔗糖合成酶是促使蔗糖进入各种代谢途径的关键酶之一。从GenBank中筛选出两条与一个蔗糖合成酶基因高度相似的EST序列,利用RACE技术获得1个长度为2 970 bp,包含完整编码框(2 448 bp)的cDNA,命名为SoSuS1(GenBank登录号:JX416283)。氨基酸序列同源性比对显示SoSuS1蛋白是一个典型的胞质内可溶性蔗糖合成酶。组织表达特异性分析显示SoSuS1在茎、叶鞘和叶片中均有表达,表明该基因在多个组织器官中发挥作用;而SoSuS1在茎中的表达量最高,茎可能是其主要作用部位。

In plants, sucrose synthase is one of key enzymes for urging the sucrose into various metabolic pathways. Two EST sequences which were highly similar to sucrose synthetase genes were screened from GenBank, and the corresponding cDNA was cloned by the technologies of RACE. The cloned sucrose synthase cDNA, named SoSuS! was 2 970 bp long （ GenBank accession No. JX416283 ） , containing the complete coding sequence （ 2 448 bp ） . The result indicated that SoSuS1 protein is a typical soluble synthetase in the cytoplasm by homoge-neous comparing of its amino acids sequences. Gene expression analysis showed that SoSuS1 was expression in stem, leaf sheath and leaf, indicating the gene plays a role in multiple tissues. The expression of SoSuS1 gene was highest in stem, and the stem was possible the main action site.