摘要
以牛睾丸为原料,对纯化后玻璃酸酶的酶学性质进行研究。结果表明,牛玻璃酸酶水解透明质酸钠的最适pH和温度分别为4.5、37℃,在4-45℃酶活稳定性较好。Nacl和BSA浓度分别为0.2、0.45mol/L时具有较高的酶活力。金属离子抑制该酶活力的顺序为,Fe3+〉Mn2+〉Zn2+〉Al3+〉Ca2+〉K+〉Mg2+。硫酸软骨素A浓度为1.2mg/mL时,酶活损失31.08%。硫酸软骨素B浓度为0.012mg/mL时,酶活增加1.36%。硫酸软骨素c浓度为1.2mg/mL时,酶活损失45.07%。肝素在0.012mg/mL时对酶存在抑制作用。人类血清蛋白在0.012mg/mL时可增加1.05%的酶活。半胱氨酸使酶失活。
The enzyme used in this study was a partially purified sample of hyaluronidase,extracted and purified from bovine testicular. Using sodium hyaluronate as substrate,the pH optimum for the enzyme was pH4.5 and maximum activity was obtained at 37℃. The enzyme also required NaCI and BSA at a concentration of 0.2mol/L and 0.45mol/L in the final incubation mixture for optimum activity. The hyaluronidase from bovine testicular was shown to be heat instable,and inhibited by metal ions,the inhibiting capacity was Fe3+〉Mn2+〉Zn2+〉Al3+〉Ca2+〉K+〉Mg2+. Chondroitin-sulphate A and C was inhibitor of bovine hyaluronidase,causing up to 31.08% and 45.07% inhibition at 1.2mg/mL concentration. The enzyme activity was enhanced 1.36% and 1.05% by chondroitin-sulphate B and human serum protein at 0.012mg/mL concentration,respectively. Heparin inhibited enzyme activity at 0.012mg/mL concentration. Cysteine completely inhibited enzyme activity.
出处
《食品工业科技》
CAS
CSCD
北大核心
2013年第22期161-164,共4页
Science and Technology of Food Industry
基金
国家现代农业(肉牛牦牛)产业技术体系项目资助(CARS-38)
国家公益性行业(农业)科研专项经费资助(201203009)
关键词
牛睾丸
玻璃酸酶
酶学特性
bovine testicular
hyaluronidase
enzymatic characteristics