期刊文献+

用凝胶电泳法研究羟基肉桂酸衍生物对DNA的辐射保护效应

GEL ELECTROPHORESIS STUDY OF RADIOPROTECTIVE EFFECT OF HYDROXY CINNAMIC ACID DERIVATIVES ON DNA
下载PDF
导出
摘要 用凝胶电泳法研究了DNA在γ射线作用下链断裂和链交联及保护剂对它的保护效应。DNA样品经电泳、荧光染色、紫外灯下照相后对底片进行光密度分析,最后经计算机数据处理,可以得到其辐照前后的分子量分布变化,并由此得出DNA的链断裂和链交联的定量数据。以此研究了冰冻干燥二元体系中羟基肉桂酸衍生物,在室温下对小牛胸腺DNA的辐射保护效应。测量了DNA与咖啡酸及芥子碱在不同摩尔比下时DNA的链交联和链断裂G值,发现两者对DNA均有明显的辐射保护效应,但咖啡酸的辐射保护效应强于芥子碱。对照对它们的ESR研究,并分析它们的结构异同,初步探讨了两者辐射保护效应强弱的原因。 ABSTRACT The radioprotective effect exerted by hydroxy cinnamic acid derivati-ves on the radiation damage of DNA, such as double-strand break (DSB). intermole-cular crosslinking (CL), has been studied by gel electrophoresis. The molecular lengthdistribution of DNA can be measured by this method, and by comparing average mo-lecular length of DNA before and after irradiation, the frequency of DSB and CL canbe determined quantitatively, The radioprotective effect of caffeic acid and sinapine oncalf thymus DNA in binary system at room temperature have been determined. Bothcaffeic acid and sinapine reveal apparent protective effects and the former is strongerthan the latter. The mechanism of radioprotection at room temperature has been discus-sed. Based. on this finding, combined with the previous ESR study and an analysis ofthe molecular structure about these two protectors, it is suggested that the target isprotected as a result of hydrogen atom transfering from protector to target.
出处 《辐射研究与辐射工艺学报》 CAS CSCD 北大核心 1991年第3期129-133,共5页 Journal of Radiation Research and Radiation Processing
关键词 脱氧核糖核酸 苯基丙烯酸 凝胶电泳 DNA Hydroxy cinnamic acid Radioprotection Gel electrophoresis
  • 相关文献

参考文献5

  • 1赵蓉,林念芸,张加山.N_2O饱和水溶液体系中胸腺嘧啶的辐射交联[J].辐射研究与辐射工艺学报,1990,8(1):48-53. 被引量:3
  • 2林念芸,辐射研究与辐射工艺学报,1986年,4卷,4期,29页
  • 3陈懿平,1986年
  • 4林念芸,辐射研究与辐射工艺学报,1983年,1卷,1期,26页
  • 5团体著者,分子放射生物学,1970年

共引文献2

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部