油松花粉蛋白提取工艺的优化

Optimization of extraction conditions for protein from pine pollen

为了充分利用油松花粉中的蛋白质,对油松花粉的基本组分进行测定,采用碱提酸沉法提取油松花粉中的蛋白,通过正交实验确定出油松花粉蛋白的最佳提取条件。结果表明,油松花粉中水分、灰分、蛋白质、脂肪及还原糖含量分别为8.72%、3.19%、20.32%、2.84%、24.71%。油松花粉蛋白的等电点pI为3.6,在此基础上研究油松花粉在料液比、提取时间、提取温度、提取pH对油松花粉蛋白提取率的影响。经过单因素实验筛选较优水平后,设计正交实验,采用极差分析和方差分析,得出油松花粉蛋白的最佳提取条件为:料液比为1∶40(g/mL),提取pH为11,提取时间为80min,提取温度为55℃,在此提取条件下,油松花粉蛋白的提取率为56.3%。

In order to make full use of protein in the pollen,and to determine the basic set of pine pollen,the alkali extraction of pine pollen in protein extraction and acid precipitation were studied. The orthogonal experiment to determine the best extraction conditions of pine pollen protein solution. Barley malt roots contained 20.32% protein,3.19% ash,8.72% water,2.84% fat and 24.71% reducing sugar content. Pollen protein isoelectric point of pl was 3.6,on this basis to research in solid-liquid ratio,alkali extraction time,alkali extraction temperature, alkali extraction rate for the effect of pH on pollen protein extraction. Through single factor experiment to select the optimum level,to design orthogonal experiment,to apply the range analysis and variance analysis,and reached a conclusion of the optimum extraction conditions of pine pollen protein which was the ratio of material to liquid to 1：40（g/mL）,alkaline extraction pH was 11 ,alkali extraction time was 80min,the temperature alkaline extraction for the 55℃. Under these extraction conditions ,the extraction rate of pine pollen protein was 56.3%.