益生菌微胶囊二次包衣工艺的优化

Optimization of preparation for double-coated probiotic microcapsules

为了进一步提高蛋白质微胶囊对两歧双歧杆菌(Bifidobacterium bifidum)在人体胃液中的保护效果,以海藻酸钠为壁材对蛋白质微胶囊进行二次包衣。通过考查两歧双歧杆菌在模拟胃液(SGJ)中的存活率和微胶囊粒径,得到了海藻酸钠对蛋白质微胶囊的最佳包衣工艺:海藻酸钠溶液浓度为0.5%,CaCl2浓度为0.05mol/L,pH为5.5,搅拌速度为500r/min。所得微胶囊平均粒径为133μm,它们能将Bifidobacterium bifidum在模拟胃液中2h后的存活率提高至14.5%。

In order to further enhance the protective effect of protein-based microcapsules on Bifidobacterium bifidum in simulated gastric juice,alginate was selected as a double-coating material. It was observed that the optimal coating process condition was obtained by evaluating the particle size of microcapsules and survival rate of microencapsulated Bifidobacterium bifidum in simulated gastric juice,and that the optimal coating process condition was..the concentration of alginate solution 0.5% ,the concentration of CaCI2 0.05mol/L, pH5.5, the agitation rate 500r/rain. The particle size of microcapsules was 133~m,and the survival rate of Bifidobacterium bifidum in simulated gastric juice was improved to 14.5% at the optimal coating condition.