珠母贝糖胺聚糖抗肿瘤作用初步研究

Primary study on the antitumor effect of glycosaminoglycan extracted from Pinctada martensii Dunder

目的：初步探讨珠母贝糖胺聚糖抗肿瘤作用。方法：采用MTT法检测珠母贝糖胺聚糖对人宫颈癌Hela细胞、小鼠白血病L1210细胞和人慢性粒细胞白血病K562细胞生长的抑制作用。采用动物体内模型检测马氏珠母贝糖胺聚糖对荷L1210小鼠生命延长率的影响。结果：珠母贝糖胺聚糖PGI、PGII在25～100μg·mL^-1浓度范围内对三种肿瘤细胞有显著抑制作用。在100μg·mL^-1时PGI、PGII对小鼠白血病L1210细胞作用最强，抑制率分别达到48．2％与40．4％；PGII与抗癌药物（5-Fu、DDP）合用时对人宫颈癌Hela细胞、小鼠白血病L1210细胞有增敏作用；珠母贝糖胺聚糖具有延长荷L1210小鼠生命的作用，其中100mg·kg。剂量组的生命延长率达19.3％。

Objective：To study anti-tumor activities of the Dunder. Methods：MTT assay was used to determine the glycosaminoglycan extracted from Pinctada martensii inhibition effects of the glycosaminoglycan called PGI and PGII extracted from Pinctada martensii Dunder on proliferation of human Hela cell,L1210 cell and K562 cell in vitro, and the ratio of extending viability on the L1210 tumor-bearing mouse caused by the glycosaminoglycan extracted from Pinctada martensii Dunder was examined in vivo. Results：MTT showed that all the PGI and the PGII inhibited the growth of human Hela cell,L1210 cell and K562 cell remarkably in a dose of 25~100μg·mL^-1. The strongest inhibitory effect of the PGI and the PGII was on L1210 cell at the dose of 100μg·mL^-1,and the maximum inhibitory ratio were 48.2% and 40.4% respectively. PGII combination with chemotherapeutic drug（5-Fu or DDP） can sensitize chemotherapeutic drugs on the HeLa and L1210 tumor. In this paper the life prolongation effect on the L1210 tumor-bearing mouse of the glycosaminoglycan had been proved ,the survival prolongation rate of the 100mg.kg-1 dosage groups was 19.3% in our experiment.