海藻玉壶汤对N-甲基亚硝基脲诱导小鼠胸腺淋巴瘤的疗效研究

Therapeutic effect of Haizao Yuhu decoction on signs of thymic lymphoma induced by N-methyl-N-nitrosourea in mice

目的研究N-甲基亚硝基脲诱导胸腺淋巴瘤小鼠的体征,观察海藻玉壶汤对小鼠症状及肿瘤进展的影响。方法每周1次、连续5周给小鼠腹腔注射N-甲基亚硝基脲建立小鼠胸腺淋巴瘤模型,观察诱癌过程中小鼠外观和外耳微循环变化,用血液流变学指标评价N-甲基亚硝基脲诱导胸腺淋巴瘤小鼠的体征,并在模型建立同时用海藻玉壶汤进行治疗,考察其对小鼠症状及肿瘤进展的影响。结果 N-甲基亚硝基脲诱导胸腺淋巴瘤过程中小鼠逐渐出现畏寒喜暖、踡缩少动、弓背毛松、外耳和尾部血管紫暗现象,同时全血黏度及红细胞聚集指数增高。海藻玉壶汤可以减弱上述体征,阻止肿瘤进展。结论 N-甲基亚硝基脲诱导的胸腺淋巴瘤小鼠表现为痰凝血瘀体征,海藻玉壶汤能改善小鼠症状和体征,阻止肿瘤进展。

[ Objective ] To study signs of thymic lymphoma induced by N-methyl-N- nitrosourea（MNU） in mice, and observe therapeutic effect of Haizao Yuhu decoction （HYD） on nmuse symptoms and tumor progress. [Methods ] The mice were injected peritoueal/y with MNU once weekly for five weeks to induce thymic lymphoma model. The changes in appearance and auricle microcireulation were observed in carcinogenic process. The signs of mice with thymic lyrnphoma were evaluated by hemorrheology. HYD wel~ used to treat mouse and investigate its effects on mouse symptoms and tumor progress when the tumor model was induced. [Results] The model mice with thymic ]ymphoma induced lay MNU appeared gradually chill, clump and lazy, hunched, cyanosis in auricle and tail. Mean- while, whole blood vis~＇osity and erythrocytie aggregate index increased. HYD showed a reduction in these signs and suppressed tumor progress. [Conclusions] The mice with thymic lymphoma induced by MNU appeared a signs of phlegm coagulation and blood stasis, the treatment with HYD could improve mouse symptoms and signs and prevent tumor progress.