Tc17细胞在结核患者外周血及胸腔积液中检测的意义

Significance of Tc17 cells in peripheral blood and pleural effusion with tuberculosis patients

目的探讨Tc17细胞在结核患者外周血及胸水中的表达及临床意义。方法应用流式细胞仪分析20例健康对照者、20例活动性肺结核患者及19例结核性胸膜炎患者外周血及胸腔积液中Tc17细胞占CD8+T细胞的比例,ELISA检测外周血血浆中IL-17A的含量。结果结核性胸腔积液中Tc17细胞占CD8+T细胞的比例(2.13±0.23)%高于同源外周血(1.15±0.17)%,且外周血中比例高于单纯肺结核患者(0.68±0.11)%及健康者外周血中比例(0.49±0.22)%,治疗后(0.63±0.15)%Tc17细胞在外周血的表达较治疗前(0.87±0.31)%下降,ELISA结果与流式结果一致。结论 Tc17参与了结核病的发生发展,并与疾病转归相关,对患者的临床指导用药及疗效观察有意义。

[ Objective ] To investigate the peripheral blood and pleural effusion expression of Tc 17 cell in patients with tuberculosis. [Methods] Intraeelluar staining and flow cytometry analysis were used to evaluate the proportion of Tcl7/ CD8＋T cells in peripheral blood and pleural effusion samples from 20 healthy donets, 20 individuals with active tuberculosis,19 individuals with pleural tubereulosis, and the content of IL-17A in peripheral blood serum were detected by ELISA. [ Results ] Tc17/CD8＋T cells in tuberculosis pleural effusion （2.13±0.23）% was higher than homologous peripheral blood （1.15±0.17）%, and this proportion in peripheral blood was higher than patients with puhnonary tuberculosis （0.68±0.11）% and healthy donors （0.49±0.22）%. After treatment the expression of Te17/ CDS＋T cells （0.63±0.15）% in peripheral blood was decreased than before （0.87±0.31）%. [ Conclusions ] Tc17 cells were involved in the occurrence and development of tuberculosis, and were associated with the prognosis of the dis- ease, it has significance for clinical medicine and curative effect observation of patients.