摘要
目的:比较研究8-异戊烯基柑橘素和柑橘素对体外培养大鼠股骨组织代谢活性的影响。方法:体外分离培养大鼠股骨组织,然后随机分为4组,其中3组分别给予雌二醇(终浓度为1×10-8mol·L-1)、8-异戊烯基柑橘素(终浓度为1×10-5mol·L-1)和柑橘素(终浓度为1×10-5mol·L-1),另外1组加生理盐水作为对照。分别在培养的第3、6、9、12、15和18 d测定大鼠股骨组织中碱性磷酸酶活性和钙含量,并采用RT-PCR检测骨保护素、Ⅰ-型胶原、Runx-2和骨形态发生蛋白基因的mRNA表达水平。结果:8-异戊烯基柑橘素、柑橘素和雌二醇均可提高股骨组织中碱性磷酸酶的活性,增加钙含量,并上调opg、bmp-2、collagen-I和runx-2 mRNA的表达水平;8-异戊烯基柑橘素活性强于柑橘素。结论:8-异戊烯基柑橘素和柑橘素均能改善体外培养大鼠股骨组织的代谢活性。
Objective： To compare the effects of 8-prenylnaringenin and naringenin on metabolic activity of rats femoral tissue in vitro. Methods： The rat femoral tissue was isolated in vitro, and randomly divided into four groups, including estrogen （ 1 ×10^-8mol·L^-1） group, 8-prenylnaringenin （ 1 ×10^-5mol·L^-1） group, naringenin （1 ×10^-5mol·L^-1） group and control group. After administered with corresponding drugs, the rat femoral tissue in each group was cultured. On d3, d6, d9, d12, d15 and dlS, the alkaline phosphatase （ALP） activity and calcium content of the rat femoral tissue were determinated. Moreover, the mRNA expression of osteoprotegerin （OPG）, type I-collagen （ collagen- I）, Runx-2 and bone morphoge- netic protein （BMP-2） was also determined by RT-PCR. Results： 8-prenylnaringenin, naringenin and estrogen can increase the ALP activity and calcium content. They can also up-regulate the mRNA expression levels of opg, bmp-2, collagen-I and runx-2. The pharmacological activity of 8-prenylnaringenin was shown to be stronger than that of naringenin. Conclusion： 8-Prenylnaringenin and naringenin can both significantly improve the metabolic activity of rat femoral tissue in vitro.
出处
《药学进展》
CAS
2013年第10期528-533,共6页
Progress in Pharmaceutical Sciences
基金
国家自然科学基金资助项目(No.81270963)
甘肃省科技重大专项计划(No.092NKDA025)
