一株沙漠小球藻不同培养方式的比较

Comparison of different culture methods for a desert chlorella

选取了一株从古尔班通古特沙漠分离的小球藻GTD8A1，考察了其在不同碳源下的异养能力以及不同培养方式下的生长情况和蛋白质、总脂含量以及脂肪酸组成的变化。结果表明，此株小球藻分别添加5种碳源时，在不同培养方式下均能生长，其中以葡萄糖和乙酸钠为外加有机碳源时生长能力最好，异养和兼养的生长周期比自养的生长周期缩短60％～80％，生物量比自养提高2—4倍。在以乙酸钠为碳源异养培养时，其蛋白质含量和脂质含量均比自养培养的高出1．5—2倍。气相色谱分析显示，其自养、兼养和异养培养时C18：3含量很高，尤其是自养时其含量达总脂肪酸含量的70％左右，兼养和异养培养时饱和脂肪酸和单不饱和脂肪酸比例增大，C18：3的比例降低。

Select a chlorella GTD8A1 that were isolated from Gurbantonggut Desert to investigate its heterotrophic capacity in different carbon sources, growth condition in different culture methods and the change of protein content, total lipid and fatty acid composition. The results showed that the chlorella added with five kinds of carbon sources in different culture methods could grow re- spectively, among them, the growth ability achieved the best when glucose and sodium＇ acetate was used as organic carbon source, the growth cycle of heterotrophy and mixotrophic cultivation was shorter 3-4 times than autotrophy, the biomass was increased by 2~4 times. When using sodium acetate as the carbon source in heterotrophic culture,the content of protein and lipid was higher 1.5-2 times than autotrophy, Gas chromatography analysis showed that content of C18：3 was very high, especially in the autotrophic culture, it reached 70% of total lipid content, the proportion of saturated fatty acid and monounsaturated fatty acid increased and the C18：3 decreased while in the mixotrophic and heterotrophic culture