摘要
目的探讨树突状细胞(DC)与细胞因子诱导的杀伤细胞(CIK)共培养后对白血病细胞株K 562、淋巴瘤细胞株Raji、肺癌细胞株A 549、胃癌细胞株BCG-803的杀伤作用。方法采集健康志愿者外周血50 mL,用淋巴细胞分离液分离单个核细胞,分别用不同的细胞因子诱导,培养DC细胞与CIK细胞。然后将成熟的DC和CIK细胞混合培养,用流式细胞仪检测两组细胞中CD 3+CD 56+、CD 3+CD 8+T细胞的比例;用MTT法检测其对K 562、Raji、A 549、BCG-803细胞株的杀伤活性。结果 DC-CIK组较CIK组具有较高的CD 3+CD 56+、CD 3+CD 8+T细胞比例(P<0.05);DC-CIK、CIK对K 562、Raji、A 549、BCG-803细胞株均具有较强的杀伤作用,且随着效靶比的增加,杀伤活性逐渐增加;DC-CIK的杀伤活性较CIK明显增高(P<0.05)。结论 DC-CIK共培养体外可增强对肿瘤细胞的杀伤作用,为肿瘤的临床治疗提供了理论和实验依据。
Objective To investigate the cytotoxicity effect of dendritic cells （DC） with cytokine induced killer cells （CIK） to leukemia cell lines K 562, lymphoma cell line Raji, lung adenocarcinoma cell line A 549 and gastric cancer cell line BCG-803 after culturing together. Methods collecting 50 mL peripheral blood of healthy volunteers, we separate peripheral blood with lymphocyte separation liquid to obtain mononuclear cells,respectively, we use different cytokines to induce and culture DC and CIK cells. Then we culture the mature I3C and CIK cells together and detect the T cell subsets proportion ofCD 3＋CD 56＋and CD 3＋CD 8＋ in two groups, Cytotoxic activity against K 562, Raji, A 549 and BCG-803 cells in two groups were detected by MTT method. Results DC-CIK group had higher CD 3＋CD 56＋,CD 3＋CD 8＋T cells as compared with CIK group （P〈0.05）; DC-CIK, CIK had strong killing effect on K 562, Raji, A 549, BCG-803 cell lines; with effect-target ratio increasing, the killing activity increased gradually; the killing activity of DC-CIK was significantly higher than the CIK （P〈0.05）. Conclusion DC-CIK cultured in vitro can enhance the killing effect on tumor cells; which provides theoretical and experimental basis for the clinical therapy for tumor.
出处
《当代医学》
2013年第30期21-23,共3页
Contemporary Medicine
