食管癌及癌前病变组织中可激发的一氧化氮合成酶的表达

Expression of Inducible Nitric Oxide Synthase in Human Esophageal Biopsies from Carcinoma and Precancerous Lesions

目的探讨可激发的一氧化氮合成酶 (iNOS)在食管癌发生发展过程中的表达及其意义。方法应用免疫组化技术检测主要来自食管癌高发区河北磁县的食管活检组织标本中 iNOS的表达。结果在正常粘膜、单纯增生、不典型增生Ⅰ、Ⅱ、Ⅲ级、原位癌、鳞癌及腺癌组织中 iNOS表达的阳性率分别为 0(0/25)、 2.5％ (1/40)、 4.0％ (2/50)、 7.5％ (6/80)、 2.5％ (1/40)、 1.4％ (1/69)、 8.5％ (3/35)及 71.4％ (25/35)。除腺癌与正常粘膜组织间 iNOS的表达差异有显著性外 (P< 0.001)，其它组织与正常粘膜组织间差异均无显著性 (P >0.1)。结论 iNOS可能与食管腺癌的发生有关，而与鳞癌的发生发展无关。

To investigate the possible role of inducible nitric oxide synthase (iNOS) in esophageal carcinogenesis. Methods iNOS expressions in normal epithelial cells(NC), hyperplastic cells(HC), atypical hyperplastic cells(AHC) from gradeⅠ toⅢ ,carcinoma in situ(CIS), squamous cell carcinoma(SCC),and adenocarcinoma(ADC) were detected with immunohistochemistry. Results The positive rates of immunohistochemistry staining was 0 for NC, 2.5％ for HC, 4.0％ ,7.5％ and 2.5％ for AHC gradeⅠ toⅢ respectively, 1.4％ for CIS, 8.5％ for SCC and 71.4％ for ADC. Conclusions There is a high expression of iNOS in human esophageal adenocarcinomas. Frequent expression of iNOS may be a potential event in ADC carcinogenesis. There is no correlation between iNOS and SCC carcinogenesis.