Ⅱ型胶原自身抗体与类风湿性关节炎的关系

The relationship between antibody to type Ⅱcollagen and rheumatoid arthrits

我们用改良的酶联免疫吸附法(ELISA),分别用牛、人天然Ⅱ型胶原和用溴化氰裂解的人Ⅱ型胶原作为包被抗原,检测了112例类风湿性关节炎患者和154例健康对照血清中抗Ⅱ型胶原的IgG型和IgA型抗体。抗天然牛Ⅱ型胶原自身抗体IgG型和IgA型阳性率RA患者组分别为19.6％和12.5％,而健康对照组为0.7％和2.0％,抗天然人Ⅱ型胶原自身抗体IgG型和IgA型阳性率RA患者分别为12.5％和16.3％,而健康对照为0％和2.5％,两类抗体的阳性率均为RA患者组显著高于对照组。用牛和人的Ⅱ型胶原作为包被抗原,其阳性率无显著性差异。这也说明了Ⅱ型胶原蛋白在种属间的抗原决定簇相似。用溴化氰裂解的人Ⅱ型胶原作为包被抗原,RA患者和健康对照组在IgG型有显著性差异,而在IgA型无统计学差异,其原因有待进一步探讨。本文根据这些结果讨论了Ⅱ型胶原自身抗体在RA发病中的意义。

An improved enzvme - linked immunosorbent assay (ELISA) for detennination of antibody to native type Ⅱ collagen in hunmn serum has been developed. Various species of type Ⅱcollagen including bovine, human collagen and Cyanogen bromide peptides of human type Ⅱ collagen were coated as antigens. 112 sera frora patients with rheumatoid arthritis (RA) and 154 sera from health control were measured. The IgG and IgA subclass of antibodies to native bovine typeⅡ collagen were present in sera of 19. 6% and 12. 5% of patients with RA, 0. 7% and 2. 0% of control sepa-rately. The IgG and IgA subclass of antibodies to native human type Ⅱ collagen were present in sera of 12. 5% and 16. 3% of patients with RA, 0% and 2. 5% of control separately.. The freo,uency of IgG and IgA antibodies to either bovine or human collagen were significantly greater in RA than that in control, but there was no significant difference between bovine and human collagen. These findings indicate type Ⅱ collagen is similar in antigen determiner. However, it is un-clear that why there was significant difference between RA and control in IgG subclass, but not in IgA subclass, when Cyanogen bromide peptides of human type Ⅱ collagen was used as coated antigen, The possible role of antibody to native type Ⅱ collagen in RA was discussed.