细菌培养中注射器连续稀释法的研究

Disquisition of a new Quantitative Dilution Method for Bacterial Culture.

目的探讨使用注射器金属棒磁力搅拌连续稀释法 (注射器装入灭菌金属小棒吸取标本后在磁力搅拌器上连续搅拌稀释 )在微生物检测中应用的优缺点。方法应用我们建立的稀释方法和传统方法对已知浓度菌株 :大肠杆菌 ,粪链球菌 ,婴儿双歧杆菌、嗜酸乳杆菌、脆弱类杆菌以及在腹泻动物模型制备的动物肠道菌群进行分析比较。结果我们建立的稀释方法较传统方法不仅操作简单、省时、省事、省材料 ,且有重复性好、准确性高等特点 ,统计学上无差别 (P >0 .0 5)。结论注射器金属棒磁力搅拌连续稀释法值得在微生态学的正常菌群标本及临床各种细菌标本的定量分析中推广使用。

Objective To investigate the advantage of a serial dilution method in quantitative bacterial culture by inserting a small metallic stick into an injector and continuously stirring on a magnetic mixer. Method Quantitation of standard or identified bacterial strains such as E.bacillus, E.faecilis, B.infantis,L.acidophilus Bacteroides fragilis as well as intestinal flora in diarrhea animal models were compared. Result The new method is simple, convenient, economical, easy to operate and effective in quantitative analysis of various specimens. There is no statistical difference(P>0.05) between two methods. Conclusion This new method of serial dilution provides a useful tool in quantitative bacterial culture and micro flora assay.