糖尿病与原位大隐静脉病变的相关性

Research on the relationship betw een diabetes mellitus and in-situ saphenous vein disease

目的分析糖尿病对原位大隐静脉血管病变的影响并探讨肿瘤坏死因子α诱导蛋白8(TNFAIP8)家族在此过程中的作用。方法选取新鲜的大隐静脉标本(CABG术中留取),按患者健康状况分为正常对照组(NG组)和糖尿病组(DG组),检测每条血管的物理特性及血管细胞与组织学形态特征,以及TNFAIP8和肿瘤坏死因子α诱导蛋白8样因子2(TIPE2)在原位大隐静脉中的表达及分布。结果血管物理特性:组织含水量无差异(P>0.05),热皱缩温度NG组>DG组(P<0.05),断裂强度NG组>DG组(P<0.01);血管细胞与组织学形态特征:生物学稳定指数NG组>DG组(P<0.01);弹性纤维含量(RE)NG组>DG组(P<0.05);胶原纤维含量(RC)DG组>NG组(P<0.05),血管管壁僵硬度(C/E)、血管中膜结缔组织基质在环行平滑肌中的体积密度(CC/MC)DG组>NG组(P<0.01);TNFAIP8和TIPE2在各组原位大隐静脉血管中均有表达及分布,DG组>NG组。结论糖尿病对原位大隐静脉血管病变有不利影响,而TNFAIP8家族在糖尿病原位大隐静脉病变中起到积极作用。

Objective To confirm that diabetes has an influence on in-situ saphenous vein, and to explore the effects of TNFAIP8 and TIPE2 on in-situ saphenous vein of diabetes mellitus. Methods The residual fresh great saphenous veins specimen were gotten from the patients during CABG surgery, which were divided into experimental group （with type 2 diabetes mellitus, DG） and control group （without type 2 diabetes mellitus, NG）. Each specimen was 5 cm long for further experiments as follows： vascular physical properties, biological stability, microscopic structure and expression, distribution of inflammatory proteins. Results Physical properties of vessels： water content： NG〉DG（P〉0.05）; vascular heat shrink temperature： NG〉DG（P〈0.05）; vascular rupture strength：NG〉DG（P〈0.01）; vascular biology stability index： NG〉DG（P〈0.01）; elastic fiber content（RE）：NG〉 DG（P〈0.05）; collagenous fiber content（RC）： DG〉NG（P〈0.05）; tube-wall stiffness （C/E）： DG〉NG（P〈0.01）; connective tissue stroma′s volume density in circular smooth muscle （CC/MC）： DG〉NG（P〈0.01）; TNFAIP8 and TIPE2： DG〉NG. Conclusion The diabetes mellitus has an influence on in-situ saphenous vein, while the TNFAIP8 and TIPE2 have an influence on in-situ saphenous vein of diabetes mellitus.