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黑曲霉风化含钾矿石过程中碳酸酐酶和半胱氨酸合成酶基因表达量的差异 被引量：5

Differences in the gene expressive quantities of carbonic anhydrase and cysteine synthase in the weathering ofpotassium-bearing minerals by Aspergillus niger
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摘要 研究黑曲霉(Aspergillus niger)在含有可溶性钾(KCl)或只含有非可溶性钾(含钾硅酸盐矿粉)两种条件下风化含钾硅酸盐矿粉时碳酸酐酶(CA)和半胱氨酸合成酶(CysM)的基因表达差异.采用修改的Czapek培养基(用Na2HPO4替代K2HPO4)培养黑曲霉,设置A组(添加含钾矿粉不加KCl)和B组(加KCl不加含钾矿粉)两种处理方式.分别从A组和B组两种处理培养的黑曲霉菌丝体中提取mRNA,反转录后通过实时荧光定量PCR(RT-qPCR)对CA和CysM基因的转录表达进行相对定量.构建目的基因(CA和CysM)与内参基因(甘油醛-3-磷酸脱氢酶,GAPDH)的相对标准曲线,验证目的基因和内参基因扩增效率一致,从而能对目的基因表达进行相对定量.结果表明,A组条件下该菌的CA和CysM基因表达分别比B组条件下相应的基因表达上调了1.7倍和11.7倍(即A组是B组基因表达的2.7倍和12.7倍),对与这两种酶合成相关的黑曲霉风化含钾矿物可能的代谢途径与功能进行了分析.研究结果为进一步探讨黑曲霉在风化含钾矿物过程中的代谢调控作用提供了基础资料.
作者 孙蕾蕾 肖雷雷 肖波 王伟英 潘辰 王世杰 连宾
机构地区 江苏省功能微生物与功能基因组学重点实验室 中国科学院地球化学研究所
出处 《中国科学：地球科学》 CSCD 北大核心 2013年第11期1828-1846,共19页 Scientia Sinica(Terrae)
基金 国家自然科学基金项目(批准号:41173091) 国家重大科学研究计划(编号:2013CB956702) 江苏高校优势学科建设工程项目资助
关键词 黑曲霉 含钾矿物 钾长石 碳酸酐酶 半胱氨酸合成酶 实时荧光定量PCR
分类号 Q933 [生物学—微生物学]
  • 相关文献

参考文献33

  • 1张涛,林间,高金耀.90Ma以来热点与西南印度洋中脊的交互作用:海台与板内海山的形成[J].中国科学：地球科学,2011,41(6):760-772. 被引量：12
  • 2Baker E T, Edmonds H N, Michael P J, et al. 2004. Hydrothermal venting in magma deserts: The ultraslow-spreading Gakkel and Southwest Indian Ridges. Geochem Geophys Geo syst, 5: Q08002, doi: 10.1029/2004GC000712.
  • 3Cande S C, Kent D V. 1995. Revised calibration of the geomagnetic polarity timescale for the late cretaceous and cenozoic. J Geophys Res, 100: 6093-6095.
  • 4Cannat M, Rommevaux-Jestin C, Sauter D, et al. 1999. Formation of the axial relief at the very slow spreading Southwest Indian Ridge (49 to 69E). J Geophys Res, 104:21825-21843.
  • 5Cannat M, Sauter D, Mendel V, et al. 2006. Modes of seafloor generation at a melt-poor ultraslow-spreading ridge. Geology, 34:605-608.
  • 6Charvis P, Recq M, Operto S, et al. 1995. Deep structure of the northern Kerguelen Plateau and hotspot related activity. Geophys J Int, 122: 899-924.
  • 7Chen Y J, Lin J. 1999. Mechanisms for the formation of ridge-axis topography at slow-spreading ridges: A lithospheric-plate flexural model. Geophys J Int, 136:8-18 Curray J R, Munasinghe T. 1991. Origin of the Rajmahal Traps and the 85E Ridge: Preliminary reconstructions of the trace of the Crozet hotspot. Geology, 19:1237-1240.
  • 8Debayle E, Sambridge M. 2004. Inversion of massive surface wave data sets: model construction and resolution assessment. J Geophys Res, 109: B02316, doi: 10.1029/2003JB002652.
  • 9Dick H J B, Lin J, Schouten H. 2003. An ultraslow-spreading class of ocean ridge. Nature, 426:405-412.
  • 10Dziewonski A, Anderson D. 1981. Preliminary reference Earth model. Phys Earth Planet Int, 25:297-356.

二级参考文献52

  • 1R. Mjelde,J. I. Faleide.Variation of Icelandic and Hawaiian magmatism: evidence for co-pulsation of mantle plumes?[J]. Marine Geophysical Researches . 2009 (1)
  • 2Paul Wessel.An Empirical Method for Optimal Robust Regional-Residual Separation of Geophysical Data[J]. Mathematical Geology . 1998 (4)
  • 3Van Ark E,Lin J.Time variation in igneous volume flux of the Hawaii-Emperor hot spot seamount chain. Journal of Geophysical Research . 2004
  • 4Maia M,Goslin J,Gente P.Evolution of the accretion processes along the Mid-Atlantic Ridge north of the Azores since5.5Ma:An insight into the interactions between the ridge and the plume. Geochemistry Geophysics Geosystems . 2007
  • 5Vidal V,Bonneville A.Variations of the Hawaiian hot spot activity revealed by variations in the magma production rate. Journal of Geophysical Research . 2004
  • 6Adam C,Vidal V,Escartín J.80-Myr history of buoyancy and volcanic fluxes along the trails of the Walvis and St.Helena hotspots (South Atlantic). Earth and Planetary Science . 2007
  • 7Meyzen C M,Ludden J N,Humler E,et al.New insights into the origin and distribution of the DUPAL isotope anomaly in the Indian Ocean mantle from MORB of the Southwest Indian Ridge. Geochemistry Geophysics Geosystems . 2005
  • 8Font L,Murton B J,Roberts S,et al.Variations in melt productivity and melting conditions along SWIR (70°E–49°E):Evidence from olivine-hosted and plagioclase-hosted melt inclusions. Journal of Petrology . 2007
  • 9Tao C,Lin J,Guo S,et al.First discovery and investigation of a high temperature hydrothermal vent field on the ultraslow spreading Southwest Indian Ridge. EOS Trans AGU . 2007
  • 10Goslin J,Patriat P.Absolute and relative plate motions and hypotheses on the origin of five aseismic ridges in the Indian Ocean. Tectonophysics . 1983

共引文献11

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中国科学：地球科学
2013年 第11期

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