人子宫内膜基质细胞的体外蜕膜化及其与腺体细胞的关系

An in vitro Model for Decidual Reaction of Human Endometrium and the Relationship between Decidualization & Gland

体外分离、培养人子宫内膜基质细胞和腺体细胞，待基质细胞生长融合后，加入孕酮刺激，使其蜕膜化。结果显示，孕酮（ｐｒｏｇｅｓｔｅｒｏｎｅ，Ｐ）组基质细胞胞体变大、变圆，尤以含胎牛血清（ｆｅｔａｌ ｂｏｖｉｎｅ ｓｅｒｕｍ，ＦＢＳ）组显著。用酶免疫分析法检测培养液中催乳素（ｐｒｏ－ｌａｃｔｉｎ，ＰＲＬ）含量发现，培养ｄ１７～２０ＰＲＬ分泌量达到高峰；免疫细胞化学染色可见基质细胞ＰＲＬ免疫反应阳性。在此基础上，将同期培养的腺细胞匀浆液加入到已蜕膜化的基质细胞中，结果发现，在加入腺体匀浆液３ｄ后蜕膜细胞胞体变小，ＰＲＬ分泌量减少，而对照组的ＰＲＬ产量则递增。结果提示，子宫内膜腺细胞对蜕膜反应有一定的抑制作用，其作用方式和机制有待于进一步探讨。

The stromal cells and glands of human endometrium were isolated and cultured. After stro- mal cells had been confluent, they were induced decidualization by P stimuli. The results showed that the stromal cells in the P-treated group became larger and ovoid, especially in the FBS group. The peak PRL level in the group P was obtained at the 17th ~19th d. Immunocy- tochemical staining for PRL showed that the strong immunoreactive signal was obtained in this time group. After establiment in vitro decidualization of stromal cells, the homogenate from glan- dular epithelial cells was added into the decidual cell. The results revealed that the decidual cells became smaller when the homogenate was added for 3 days ,the PRL production in the 1μg/ml group was decreased but the PRL content in the control group was gradually augmented. These findings suggest that the decidualization of stromal cells can be inhibited by the glandular epithe- lial cells, and the pattern and mechanism need to be further studied.