系统性念珠菌感染小鼠动物模型的建立

Research of Murine Model of Systemic Candida Albicans Infection

目的建立稳定的系统性念珠菌感染小鼠动物模型。方法将健康雄性昆明小鼠随机分成3组,实验组1经鼠尾静脉注射相同数量的对数生长期白念珠菌酵母细胞(2×107CFU/mL,0.5mL),实验组2经鼠尾静脉注射相同数量的对数生长期白念珠菌酵母细胞(2×106CFU/mL,0.5mL),正常对照组注射0.5mL的PBS磷酸盐缓冲液。进行小鼠死亡率观察;小鼠脾脏组织白念珠菌培养、鉴定;将肾脏组织做病理学标本PAS染色。结果实验组小鼠的死亡率为100%,高浓度组3天内全部死亡,低浓度组生存期稍长,但观察期间也全部死亡;两实验组脾组织培养有白念珠菌生长;肾组织病理学检查发现炎性肉芽肿形成,肉芽肿内有大量的白念珠菌孢子和菌丝生长及炎细胞浸润。结论直接将适量的白念珠菌酵母细胞经尾静脉注射入小鼠体内可以建立稳定的系统性念珠菌病小鼠模型,避免了应用免疫抑制剂所导致的人为性实验干扰因素,更好地为系统性念珠菌病的研究提供了动物模型。

Objective To establish a stable murine model of systemic candidiasi~. Methods Mice were randomly di- vided into 3 groups. The study group I and study group 2 were challenged intravenously via the lateral tail vein with Candida albicans 2 x 107 CFU/mL O. 5mL and 2 x 106 CFU/mL O. 5ml, respectively. The cnntr,1 group was given PBS 0.5mL. Mortality were track recorded. Colony forming units（CFUs） of C. alhicans were determined in infected spleens with plating dilution method, Histopathological changes of infected kidneys were measured. Results The mortality of two study groups were 100% while the higer doge group died with- in 3 days. In contrast, all mice infected with PBS survived throughout the experiment. CFUs of C. albicatts were significantly higher in spleen in the 2nd and 3rd day after infection in the study gronp than those in the control group（PBS）（P 〈 0.05 ）. Histological examinations of kidney tissue revealed that two st,dy gro,ps formed more extensive hyphae and fungal cells with centripetal spreading into the medulla. Candida cells formed bundles within tubule lumens. Extensive accumulations of neatrnphilic granulocytes were often observed associated with fungal colonies. Conclusion Challenging intravenously via the laleral tail vein with C. olbicwts directly without immunosuppressive agents can established a stable murine model of systemic caudidiasis.