GPR34在原发性肝细胞癌中表达的临床意义

The expression of G-protein coupled receptor in hepatocellular carcinoma

目的探讨G蛋白偶联受体34（G—proteincoupledreceptor34，GPR34）的表达与肝细胞癌发生发展的相关性。方法应用肝细胞癌组织芯片和免疫组织化学技术，检测GPR34蛋白在44例肝细胞癌组织中的表达，X2检验和Fisher检验及Spearman相关检验，分析GPR34表达与肝细胞癌临床病理特点的相关性，应用Kaplan—Meier方法计算肝细胞癌患者术后5年生存率及生存时间。结果GPR34在肝细胞癌组织中的表达比癌旁组织明显上调（P=0．003）；GPR34表达与肝细胞癌大小（P=0．024）、小肝癌（P=0．030）和浸润深度（P=0．012）显著相关，与性别、年龄、肝硬化、组织分化程度及临床分期无明显相关性（P〉0．05）；高表达和低表达GPR34的患者术后5年累积生存率分别为56．6％和66．0％，中位生存时间为59．0（95％凹：49．9～68．1）个月和65．0（95％CI：56．4～73．6）个月，Kaplan-Meier分析显示其差异有统计学意义（X2=5．617，P=0．018）。多因素分析显示，GPR34可以作为一个独立的预后评估因素（P=0．037）。结论GPR34在肝细胞癌组织中的表达上调，提示GPR34在其中具有促癌作用。GPR34表达上调与肝细胞癌大小、小肝癌、浸润深度和患者的生存时间相关。GPR34表达上调可以作为肝细胞癌预后不良评估的一个潜在影响因素。

Objective To determine the expression of G-protein coupled receptor 34 （GPR34） in hepatocellular carcinoma tissues. Methods Immunohistochemistry and tissue-array were used to determine GPR34 expression in human hepatocellular carcinoma tissues and matched adjacent tissues. A statistical analysis was performed to establish the potential correlation between GPR34 expression and the patients＇ clinicopathological characteristics, tumor progression, and prognosis. Results GPR34 is up-regulated in primary hepatocellular carcinoma tissues compared with matched adjacent tissues （P = 0. 003 ）, and it was shown that GPR34 expression is significantly correlated with tumor size （ P = 0. 024）, small hepatocellular carcinoma（0.030） and infiltration（P =0.012）, but not with sex, age, grade, clinical stage （all P 〉 0. 05 ） ; it was also shown that GPR34 expression had a significant influence on prognosis （ X2 = 5. 617, P = 0. 018）. Multivariate analyses showed that high GPR34 expression is an independent poor prognostic factor for overall survival （P = 0. 037 ）. Conclusions The up-regulation of GPR34 acts as an potential pro- oncogene in the development and progression of hepatocellular carcinoma. GPR34 may be a useful diagnostic or prognostic molecular biomarker, and a potential target for therapeutic intervention.