高效液相色谱法测定前胡属植物中紫花前胡苷的含量

Determination of Nodakenin in Peucedanum

目的 :建立高效液相色谱法测定前胡药材中紫花前胡苷的含量。方法 :Shim PackCLC ODS柱 ,流动相乙腈 甲醇 水 (2 0∶5∶75 ) ,紫花前胡苷为对照品 ,外标法峰面积定量 ,紫外检测 (λ =334nm)。结果 :6个不同品种前胡中的紫花前胡苷含量为 0 .0 0 5 %～ 3.0 90 %。结论 :紫花前胡苷不能作为评价前胡属植物的药用指标 ,但可以作为紫花前胡的质量控制标准。

Objective:To establish a HPLC method to determine the contents of nodakenin in Peucedanum .Method:The determination method was achieved by RP HPLC on an ODS column by UV detection at 334 nm; with nodakenin as external standard and H 2O CH 3OH CH 3CN(75∶5∶20) as mobile phase.Result:Several samples of 6 species of Peucedanum were analyzed by this method. The contents of nodakenin ranged from 0.005%～3.090%.Conclusion:Nodakenin cannot be used to control the quality of Peucedanum for pharmaceutical use, but this method is a simple and feasible way to control the quality of P. decursivum . [