东亚钳蝎毒抗癌多肽对H_(22)荷瘤小鼠免疫调节作用

Effect of Anticancer Polypeptide from Buthus Martensii Venom on Immune Function in the H_(22)-bearing Mice

目的 :探讨东亚钳蝎毒抗癌多肽 (APBMV)对H2 2 荷瘤小鼠免疫功能的影响。方法 :采用MTT比色法和血清溶血素测定法检测了经APBMV及其与 5 Fu联合用药治疗的H2 2 荷瘤小鼠的细胞及体液免疫功能。结果 :APBMV组及APBMV与 5 Fu联用组的NK细胞活性明显增高 (P <0 .0 5 ) ,ConA诱导的淋巴细胞转化指数明显增高 (P <0 .0 5 ;P <0 .0 1) ,与 5 Fu组比较差异显著 (P <0 .0 5 ;P <0 .0 1)。DNCB诱导的皮肤迟发型超敏反应表明 ,APBMV组及其与 5 Fu联用组的耳重差显著增高 (P <0 .0 1;P <0 .0 5 ) ,与 5 Fu组比较也有显著差异 (P <0 .0 1)。5 Fu组的白细胞数最低 ,与APBMV组及APBMV与 5 Fu联用组比较有显著差异性 (P <0 .0 0 1;P <0 .0 5 ) ,APB MV组的半数溶血值 (HC50 )显著增高 (P <0 .0 1)。结论 :APBMV可显著增强H2 2 荷瘤小鼠免疫功能 ,减低肿瘤或化疗所致的免疫功能低下。

Objective:To observe the effect of anticancer polypeptide from Buthus Martensii Venom (APBMV) on Immune function in the H 22 bearing mice. Methods: The MTT colorimetric method, homolysin assay, lymphocyte tsansformation test, delayed hypersensitivity assay and WBC count of peripheral blood were used in this study. Results: APBMV could obviously augement NK activity, promote proliferation of lymphcytes induced by Con A, potentiate the response of DTH induced by DNCB, antagonize the decrease of WBC in peripheral blood induced by 5 Fu in the H 22 bearing mice. Conclusion: APBMV can obviously increase immune function in the H 22 bearing mice and antagonize hypoimmunity immunodeficiency or immunodeficiency induced by chemotherapy or the tumor. [