摘要
目的 :用两种培养基对连翘的体外抗菌活性进行评价。方法 :用琼脂稀释法测定连翘对葡萄球菌的最低抑菌浓度 (MIC)。结果 :连翘水煎剂对表皮葡萄球菌的MIC90 ,用M H琼脂为 1∶6 40 ,而用营养琼脂则为 1∶40 ,使连翘的抑菌效果增加 16倍。结论 :中药体外抗菌实验应使用M H琼脂 ,淘汰使用营养琼脂。此外 ,应使用多点接种仪接种细菌。
Objective:To evaluate the antibacterial activity of Forsythia suspensa in vitro with different media.Method:MIC determination of Forsythia suspensa against Staphylococci was performed by the agar dilution method.Result:MIC 90 of decoction of Forsythia suspensa against Staphylococcus epidermidis in M H agar was 1∶640, but in nutrient agar 1∶40,the antibacterial activity with M H agar being 16 fold higher than nutrient agar.Conclusion:The M H agar should be recommended to replace nutrient agar as medium in the antibacterial experiment of Traditional Chinese medicine, and it is better to use multipoint inoculating device in the sensitivity test. [
出处
《中国中药杂志》
CAS
CSCD
北大核心
2000年第12期742-745,共4页
China Journal of Chinese Materia Medica
基金
国家中医药管理局重点资助!课题 (97Z0 99)
关键词
连翘
M-H琼脂
金黄色葡葡球菌
抗菌
Forsythia suspensa
M H agar
Staphylococcus arueus
S.epidermidis
antibacterial activity
