同种异体BMMSCs对去势山羊体内BMMSCs生物学行为影响的研究

Intravenous injection of allogeneic BMMSCs reverses the dysfunction of BMMSCs in oophorectomized femal goats

目的：观察去势山羊接受静脉注射同源异体骨髓间充质干细胞（Bone marrow mesenchymal stem cells，BMMSCs）后，其体内BMMSCs增殖及分化功能的改变。方法：取3—6岁关中雌性山羊15只，随机分为双侧卵巢切除组（OVX）、双侧卵巢切除24h后静脉注射同源异体BMMSCs组（OVX＋injection）和假手术对照（sham）组（n=5），并进行相应处理。1年后分离各组山羊的BMMSCs，MTT法检测其增殖能力；成骨、成脂诱导培养后，采用茜素红染色和油红O染色观察其多向分化能力。结果：去势1年后，OVX组与Sham组相比，BMMSCs的增殖能力和成骨分化能力均明显下降，而成脂分化能力明显增强（P〈0．05）；OVX＋injection组BMMSCs的增殖和成骨分化能力均较OVX组明显提高，而成脂分化能力明显下降（P〈0．05）。结论：静脉注射同种异体BMMSCS可逆转去势后BMMSCs的功能异常。

AIM ： To investigate the effects of intravenous injection of allogeneic bone marrow-derived mes- enchymal stem cells （BMMSCs） on the proliferation and differentiation of BMMSCs in oophorectomized femal goats. METHDOS： 15 female goats aged 3-6 years old were randomly divided into bilateral ovariectomy group （OVX）, sham group （Sham） and intravenous injection of allogeneic BYlMSCs 24 h after bilateral ovariectomy（ OVX ＋ injection） group. Goats in all groups were sacrificed 1 year after treatment. The proliferation and differentiation of BMMSCs were examined by MTT assay, alizarin red and oil red staining respectively. RESULTS ： The capabilities of proliferation and osteogenic differentiation of BMMSc in OVX group were lower than those in the sham group（P 〈 0.05 ） , but the capa- bility of adipogenic differentiation was higher（ P 〈 0.05 ）. The abilities of proliferation and osteogenic differentiation of BMMSc in OVX ＋ injection group were higher than those in OVX group（P 〈 0.05）, and the ability of adipogenic dif- ferentiation was lower（P 〈 0.05）. CONCLUSION： The dysfunction of BMMSCs in the oophorectomized femal goats can be reversed by intravenous injection of allogeneic BMMSCs.