硅化胶原对人牙髓干细胞成牙本质分化的影响

Effects of intrafibrillar silicified collagen scaffold on the odontoblastic differentiation of human dental pulp stem cells

目的:通过观察硅化胶原支架材料对人牙髓干细胞牙本质再生相关基因表达的影响,探讨其对成牙本质细胞分化和牙本质再生的促进作用。方法:体外培养人牙髓干细胞,分别与硅化胶原、胶原材料复合,构建细胞-支架复合体后,置于矿化诱导液和常规培养液中培养。分别于培养48 h和2周、3周时,扫描电镜和HE染色观察不同培养条件下牙髓干细胞在两种胶原支架材料上的生长情况;半定量PCR、实时定量PCR法比较各组DSPP、DMP-1、OCNmRNA表达水平的差异。结果:人牙髓干细胞在硅化胶原支架材料上生长情况与胶原材料组相比无明显差异;胶原支架材料经硅化处理后能明显促进DSPP、DMP-1、OCN等牙本质再生相关基因的表达。结论:胶原支架材料经硅化处理后能促进人牙髓干细胞向成牙本质细胞分化。

AIM： To investigate the effects of intrafibrillar - silicified collagen scaffolds on the odontogenic differentiation of human dental pulp stem cells （hDPSCs）. METHODS ： In vitro cultrured hDPSCs were seeded onto intrafibrillar-silicified collagen scaffold and nonsilicified collagen scaffold in MEM medium or odontogenic differentia- tion medium respectively. Scanning electron microscopy and HE staining were performed to detect the growth of hDP- SCs on the scaffolds. RT-PCR was used to evaluate the odontogenic differentiation of＇hDPSCs by using odontogenic marker genes. RESULTS ： The growth state of the hDPSCs on the nonsilicified collagen scaffold was similar to that on the intrafibrillar-silicified collagen scaffolds. Intrafibrillar-silicified collagen scaffold could significantly up-regulate the expression levels of odontogenic differentiation related genes DSPP, DMP- 1 and OCN of hDPSCs. CONCLUSION ： The intrafibrillar-silicified collagen scaffolds may promote the odontogenic differentiation of hDPSCs.