脑络欣通及其拆方对脑缺血再灌注大鼠NGF、PI3K、Akt以及Bad蛋白表达的影响

Effects of Naoluo Xintong Decoction on expressions of NGF,PI3K,Akt and Bad in rats with cerebral ischemia /reperfusion injury

目的观察局灶性脑缺血再灌注损伤大鼠缺血半暗带NGF、PI3K、Akt以及Bad蛋白的动态表达及脑络欣通(黄芪、川芎、三七、蜈蚣等)对其影响。方法以线栓法阻塞大鼠左侧大脑中动脉,复制局灶性脑缺血再灌注模型,缺血2 h再灌注3、7、14 d,用免疫组化染色SABC法分别检测缺血半暗带NGF、PI3K、Akt、Bad的表达。结果与模型组相比,脑络欣通组在脑缺血再灌注3、7、14 d后,能够明显增加PI3K、Akt的表达(P<0.01或P<0.001);在再灌注7、14 d,脑络欣通组NGF蛋白表达显著升高(P<0.05或P<0.01);再灌注14 d,脑络欣通组Bad蛋白表达显著减少(P<0.05)。结论脑络欣通能够保护脑缺血再灌注损伤大鼠脑组织,其作用机制可能与促进NGF表达,激活PI3K/Akt通路并降低Bad蛋白表达有关。

AIM To observe the dynamic expressions of NGF, PI3K, Akt and Bad in the ischemic penumbra of rats with cerebral ischemia/reperfusion （I/R） and to study the role of Naoluo Xintong Decoction （Astragali Ra- dix, Chuanxiong Rhizoma, Notoginseng Radix et Rhizoma, Scolopendrap, etc. ） in the treatment. METHODS The model of I/R was induced by the intraluminal suture method, which made its left middle cerebral artery occlu- sion for two hours. Rats were randomly divided into 3 d group, 7 d group and 14 d group after ischemia reperfu- sion. Expressions of NGF, PI3K, Akt and Bad in ischemic penumbra were detected by SABC, respectively. RE- SULTS Expression index of PI3K and Akt in Naoluo Xintong Decoction （3 d, 7 d, 14 d） groups were higher than those in the model groups （P 〈0. 01 or P 〈0. 001 ）. Protein expression index of NGF in Naoluo Xintong De- coction （7 d, 14 d） groups were higher than those in the model groups （P 〈0.05 or P 〈0.01 ）, while protein ex- pression index of Bad in Naoluo Xintong Decoction （ 14 d） group were markedly lower （ P 〈 0.05 ）. CONCLU- SION Naoluo Xintong Decoction can play a protective role in brain tissue damage induced by cerebral ischemia reperfusion. And their effcts might be correlated with enhancing the expression of NGF, activating the PI3K/Akt signaling pathway and down-regulating the expression of Bad protein.