南方水稻黑条矮缩病毒非结构蛋白的亚细胞定位研究

The Subcellular Localizations of Non-structural Proteins of Southern Rice Black-streaked Dwarf Virus

病毒蛋白的亚细胞定位可以反映病毒的某种功能,是研究病毒功能的重要依据。借助农杆菌的介导,将南方水稻黑条矮缩病毒(Southern rice black-streaked dwarf virus,SRBSDV)可能编码的6种非结构蛋白分别在本氏烟细胞中表达,通过荧光共聚焦显微镜观察所表达的蛋白亚细胞定位情况。结果表明:Pns52、Pns91定位相似,主要定位于细胞质,在细胞质中形成较大的颗粒状聚集体;Pns6、Pns71、Pns72和Pns92定位相似,分散于整个细胞,主要定位于细胞质中或膜上,均形成丝网状结构;WoLF PSORT对比分析显示它们可能都是膜内在蛋白(Integral membrane protein),此外,Pns6在细胞质中还可以形成颗粒状的聚集体。

Studies on the cellular localization patterns of a viral protein can provide clues to understand its mode of action.The six genes encoding non-structural proteins of southern rice black-streaked dwarf virus（SRBSDV） were cloned and expressed in Nicotiana benthamiana cells,respectively.Their subcellular localizations were observed by confocal fluorescent microscopy.The results showed that Pns52 and Pns91 were localized in the cytoplasm and formed large aggregates; Pns6,Pns71,Pns72 and Pns92 were localized in the cell cytoplasm and/ or on the cell membrane and all formed reticular strucures attached on cytoskeletons,and Pns6 could also form small aggregates in the cell cytoplasm.