夏枯草香附水煎剂对行为绝望抑郁模型小鼠抗抑郁作用研究

Antidepressant Effect of the Water Extract Isolated from Spica Prunella and Cyperus Rotundus L. on Depression in Mice

目的:观察夏枯草香附水煎剂(SP-CR-W)的抗抑郁作用。方法:清洁级ICR小鼠50只,随机分空白对照组、盐酸氯米帕明组、夏枯草香附水煎剂高、中、低剂量(1.288、0.644、0.322g/kg)组,每组10只,分别予相应药物灌胃,10天。通过小鼠自主活动、小鼠悬尾实验(TST)、小鼠强迫游泳实验(FST)和利血平拮抗实验,评价夏枯草香附水煎剂的抗抑郁效果。结果:夏枯草香附水煎剂高、中剂量(1.288、0.644g/kg)均能显著缩短小鼠在TST及FST的不动时间(P<0.05或P<0.01),对小鼠自主活动均无显著影响(P>0.05)。夏枯草香附水煎剂各剂量组均能拮抗利血平注射后1h所致的小鼠降温作用,减少小鼠眼睑下垂数及呆在圈内的动物数。结论:夏枯草香附水煎剂可以改善小鼠抑郁行为,具有抗抑郁作用,且无中枢兴奋性作用。

Objective：To study the antidepressant effect of Spica prunellae and Cyperus rotundus L.-water mixture （SP-CR-W） on depression mice. Methods：Fifty ICR mice were randomly divided into 5 groups as follows： blank control group,positive control group,and SP-CR-W groups（1.288,0.644,0.322 g·kg^-1）. All groups were administered intragastrically for 7 days. The tests of spontaneous motion,tail suspension（TST）,forced swimming（FST） and antago- nism of reserpine in mice were carried out to observe the antidepressant effect of SP-CR-W. Results：SP-CR-W （1.288 and 0.644 g＇kg-l）significantly shortened the dead time of tail suspension and forced swimming in mice （P〈 0.05 or P〈0.01 ） while it had no effect on spontaneous motion. SP-CR-W in different doses resisted the descent of body temperature and reduced the numbers of the drooping eyelids and staying inside the circle induced by the injection of reserpine after 1 hour. Conclusion：SP-CR-W has antidepressant effect and can relieve the depressant behaviors of mice.