基于石墨烯-金纳米粒子复合材料的DNA生物传感器

Electrochemical DNA Biosensor Based on Graphene-Gold Nanoparticles Hybrid

以柠檬酸钠为还原剂和稳定剂制备石墨烯-金纳米粒子复合材料,复合材料被柠檬酸钠羧基化后与末端氨基修饰的DNA发生键合,制备DNA探针,并以蒽醌-2-磺酸钠(AQMS)为杂交指示剂检测DNA序列的特异性.结果表明:基于石墨烯-金纳米粒子修饰的DNA传感器可选择性区分单碱基错配的目标DNA序列;该传感器具有较高的特异选择性.

The graphene-AuNPs hybrid was achieved with sodium citrate as a reductant and stabilizer and was functionalized by carboxyl group.DNA was successfully bound to the surface of the hybrid through a condensation reaction between the terminal amino group on the lysine residues of DNA and carboxyl group on AuNPs.A novel model of the DNA biosensor based on the graphene-AuNPs hybrid electrode was fabricated.The sodium 9,10-dihydro-9,10-dioxoanthracene-2-sulphonate （AQMS）was used as the hybridization indicator for the target DNA detection.The results show that the DNA biosensors fabricated with the graphene-AuNPs selectively distinguishs the single-base mismatch of target DNA sequence and shows a higher specific selectivity.