神经疾病患者外周血博尔纳病病毒磷蛋白抗体的检测

The detection of phosphoprotein antibody against the Borna disease virus in patients with neurological disorders

目的探讨贵州省部分地区博尔纳病病毒(Borna disease virus,BDV)在神经系统疾病患者中的感染情况。方法建立检测血清中BDV的免疫印迹(Western blot,WB)方法,采用WB方法检测贵州省120例神经系统疾病患者,包括病毒性脑炎(VE)患者62例,帕金森病(PD)患者32例,吉兰-巴雷综合征(GBS)患者19例,多发性硬化(MS)患者7例,以及贵州省120名健康体检者(对照组)外周血中BDV磷蛋白P24抗体(BDV-P24抗体)水平并进行分析。结果 120例神经系统疾病患者外周血中,BDV-P24抗体阳性7例(5.83%),其中VE患者4例(6.45%),PD患者2例(6.25%),GBS患者1例(5.26%),MS患者及对照组中均为阴性。神经系统疾病患者阳性率明显高于对照组(P=0.007),VE、PD患者阳性率均高于对照组(分别P=0.013,P=0.043);GBS及MS患者阳性率与对照组比较差异无统计学意义(均P>0.05)。结论贵州省有少部分神经疾病(VE、PD、GBS等)患者曾经感染过博尔纳病病毒。

Objective To study the infectious situation of Borna disease virus （BDV） in patients with neurological disorders in some areas of Guizhou province.Methods The immunoblot method of BDV antibody was established in peripheral blood.The immunoblot method （Western blot） was adopted to detect BDV antibody in serum.Phosphoprotein （P24） antibody against the BDV in peripheral blood serum was detected among 120 cases of patients with neurological disorders and 120 cases of healthy people from Guizhou Province.In 120 cases of patients with neurological disorders,there were 62 cases with viral encephalitis （VE）,32 cases with Parkinson＇ s disease （PD）,19 cases with Guillain-Barre syndrome （GBS）,and 7 cases with multiple sclerosis （MS）.The results of the experiment was analyzed.Results In the peripheral blood of 120 cases with neurological disorders,phosphoprotein antibody were detected in 7 cases,and the positive rate was 5.83%.In the 7 cases with positive samples,there were 4 cases with VE （6.45%）,2 cases with PD （6.25%）,and 1 case with GBS （5.26%）.In contrast,positive plasma samples were not detected in MS and the healthy control group.It was shown that the positive rate of patients with neurological disorders was significantly higher than that of the healthy control group （P=0.007）.The positive rates in VE and PD patients were significantly higher than that of the healthy control group （P=0.013,P=0.043 respectively）.The positive rates of BDV antibody in GBS and MS patients did not have statistically significant difference with the healthy controls （P〉0.05）.Conclusions Our data indicated that the BDV infection presented in some patients with neurological disorders （such as VE,PD,GBS,etc）.