三种群银鲫的RAPD分析初报

Preliminary report on the RAPD analysis of three populations of Carassius auratus gibelio

利用２０个随机引物对产于方正、彭泽和淇河地区的三个银鲫种群进行了ＲＡＰＤ检测，结果显示１４个引物的扩增效果良好，单一引物扩增的ＤＮＡ片段数在２－１３条之间，片段长度在３００－４０００ｂｐ之间。根据ＲＡＰＤ结果，进行了银鲫种群内、种群间的遗传差异分析。１４个引物中，引物ＯＰＶ－０７、ＯＰＶ－１４和ＯＰＶ－２０等对三种群银鲫扩增出的指纹图谱差异显著，可作为银鲫种群鉴定的分子标记。

populations of Carassius auratus gibelio lived in Fangzheng County (Heilongjiang Province), Pengze County (Jiangxi Province) and Qihe River (Henan Province) were analyzed by RAPD method using twenty arbitrary primers. The experiment shows that fourteen primers were effective, each primer gave 2 - 13 bands for the each individual. The length of the fragment was 300 - 4000bp. According to the patterns, genetic variations of inter-and intrapopulations of C. auratus gibelio were analysed. Among three populations, the differences of fingerprints obtained by primers OPV - 07, OPV - 14 and OPV - 20 were so obviously that might be used to be molecular markers.