冶炼工人染色体损伤与XRCC1基因多态关系研究

Study on the Association of the Susceptibility of Chromosomal Damage Induced by Heavy Metals with Genetic Polymorphism of XRCC1 Among Smelting Workers

目的探讨X线交叉互补基因1(XRCC1)基因多态与冶炼工人血淋巴细胞染色体损伤的关系。方法以166名冶炼工人为暴露组,98名非冶炼工人为对照组,采用PCR-RFLP技术对血细胞XRCC1基因相关多态位点进行检测。结果暴露组XRCC1 194 Arg和Trp等位基因频率分别为70.0%和30.0%,XRCC1 280 Arg和His等位基因频率分别为86.0%和14.0%,XRCC1 399 Arg和Gln等位基因频率分别为83.0%和17.0%。多因素Poisson回归分析结果表明,女性发生染色体损伤的危险性为男性的1.63倍(P<0.01),XRCC1 194 Arg/Trp、Trp/Trp基因型个体发生染色体损伤的危险性为194 Arg/Arg基因型个体的0.6348倍(P<0.05)。结论女性和XRCC1 194 Arg/Arg基因型个体可能为重金属致染色体损伤的易感人群。

Objective To explore the relationship between the genetic polymorphism of X-ray cross-complementing group 1 （XRCC1） and susceptibility of chromosomal damage in lymphocytes among smelting workers.Methods Totally,166 smelting workers were included into the exposure group and 98 non-smelting workers into the control group.PCR-RFLP technique was applied to detect the single nucleotide polymorphisms （SNPs） in XRCC1 gene （c.194、c.280 and c.399）.Results For the exposure group,the frequencies of occurrence of the Arg and Trp alleles of XRCC1 194 were 70.0 ％ and 30.0 ％and those of the Arg and His alleles of XRCC1 280 were 86.0％ and 14.0％,whereas the frequencies of Arg and Gln alleles of XRCC1 399 were 83.0％ and 17.0％.Multivariate Poisson regression analysis indicated that the risk of chromosomal damage for the female heavy-metal-exposure workers was 1.63 of that for the male workers （P ＜ 0.01）.The risks of chromosomal damage induced by heavy metals for individuals carrying XRCC1 194 Arg/Trp,Trp/Trp genotype was 0.6348 of that carrying XRCC 1 194 Arg/Arg genotype （P ＜ 0.05）.Conclusions Females and individuals carrying XRCC1 194 Arg/Arg genotype could be at higher risk of chromosomal damage when they are exposed to heavy metals.