小红参醌对低温储存皮肤活力的影响

The effect of Rubiaquinone on viability of cadaver cryopreservated skin

目的 :探讨小红参醌作为抗冻剂添加剂 ,在皮肤的低温储存中对皮肤活力折影响。方法 :皮肤的低温储存采用速冻玻璃化法和慢冻法 ,将小红参醌分别按照 0 5 μg/ml和 1μg/ml的浓度加入抗冻液中 ,每种方法分为常规组、0 5 μg/ml小红参醌组和 1μg/ml小红参醌组。利用三个实验组分别对小块离体豚鼠皮和异体尸体皮进行处理 ,然后在液氮温度下进行深低温储存。对储存后皮肤分别进行氧耗量、琥珀酸脱氢酶活性的测定 ,并计算平均活力。结果 :对于豚鼠皮和异体尸体皮 ,无论速冻法和慢冻法两种浓度的小红参醌对低温储存后皮片的活力均未发现有明显的提高。除大块异体皮慢冻组琥珀酸脱氢酶 ,小块异体皮慢冻组耗氧量之间尚有差异以外 ,其他组别氧耗量、琥珀酸脱氢酶活性测定值以及平均活力之间均无显著差异。结论 :抗氧化剂小红参醌在皮肤的低温储存中对皮肤的活力没有影响 ,表明深低温储存中过氧化损伤不同于 4℃下保存 ,可能存在其他机制 ,需要进一步研究。

Objective:To discuss the effect of Rubiaquinone on the cryopreservation of skin as a additive to cryoprotectent.Methods:Low speed cooling method and vitrification were adopted as the method of skin cryopreservation. Three group which contain none, 0 5μg/ml, 1μg/ml Ruibiaquinone were created for each method. After preserved at liquid nitrogen, the viability of small sheet of guinea pig and cadaver skin were assessed by oxygen consumption and SDH. Then average viability is also calculated.Results:.Our results showed that Rubiaquinone had no effect on whatever guinea pig and cadaver skin with low speed cooling or vitrification.Conclusion:Our data indicated that the peroxide injury in cyropreservation was different from that at 4℃. Further research should be carried out for understanding its inherent machanism.