乳胶凝集试验检测鸡新城疫病毒血清抗体的研究

Latex Agglutination Test to Detect Serum Antibodies Against Newcastle Disease Virus

用硫酸铵沉淀、透析浓缩的鸡新城疫病毒 (NDV)抗原致敏乳胶 ,然后与鸡新城疫阳性血清进行方阵滴定 ,选择出最佳致敏条件并制成新城疫病毒乳胶抗原 ,由此建立起乳胶凝集试验 (LAT) ,用来检测新城疫血清抗体 ;用所建立的乳胶凝集试验 (LAT)和血凝抑制试验 (HI)同步检测 6 9份鸡血清 ,结果乳胶凝集试验阳性 6 2份 ,阴性 7份 ;血凝抑制试验阳性 6 6份 ,阴性 3份 ,两者的总符合率为 94.2 % (6 5 / 6 9) ,且两者的检出率基本一致。结果表明 ,乳胶凝集试验具有操作简便、快速、敏感性高、特异性强且可用于现场检测等优点 。

Newcastle disease virus (NDV)was precipitated with (NH 4) 2SO 4 and concentrated after dialysis.Suitable concentration of NDV antigen determined by chequerboard titration was used to sensitize latex treated formerly and the latex antigen was prepared,so the latex agglutination test for detecting antibodies against NDV was established.We tested 69 chicken sera by LAT and HI,the amount of the positive or negative sera detected was 62,7(LAT);66,3(HI)respectively.The total coincidence rate of both methods reached 94.2%(65/69),and also positive and negative rates were almost identity respectively.The results indicated that LAT has advantages such as simple、rapid、sensitive、specific and detectable on site and so on,it is a new method that is suitable for grass-roots unit to detect serum antibodies against chicken NDV.