应用聚合酶链反应检测鉴别火鸡支原体方法的建立

Detection and Identification of Mycoplasma Meleagridis by Polymerase Chain Reaction

根据基因库中火鸡支原体 (MM) 1 6S rRNA基因序列 (GenebankU0 46 49) ,设计了一对跨幅为 85 0bp的引物 ,用这对引物对 4禽株火鸡支原体标准菌株和 6种其它禽病病原体进行PCR扩增 ,结果 4禽株火鸡支原体菌株均得到片段大小与预期设计相一致的 85 0bp的PCR扩增产物 ,而其它 6种禽病病原体 (包括MG、MS和MI)的扩增结果则均为阴性 ,该PCR能检出1 0

A set of primers,XZ19、XZ20 were designed according to thesequence of 16s rRNA gene of Mycoplasma Meleagridis (MM) from Genebank U04649. A MM specific 850_base pair DNA product was amplified by theseprimers from four MM reference strains,but not from six other avian pathogenic virus and bacteria (including MG、MS and MM). As little as 100fg ofMM DNA was detected using gel electrophroesis.