期刊文献+

原子力显微镜对不同类型胶原蛋白与细胞黏附情况的研究

The adhesion study of different types of collagen and cell by atomic force microscope
原文传递
导出
摘要 目的研究不同类型胶原蛋白与成纤维细胞及角质形成细胞的黏附情况,并用原子力显微镜观察其相互作用。方法分别用牛肌腱胶原、鼠尾胶原和标准Ⅳ型胶原包被培养板,接种相同数量的原代角质细胞,在不同时相用活细胞电子计数仪测定未黏附细胞的数量,观察不同类型胶原所未黏附的细胞数量的多少,并用原子力显微镜观察胶原蛋白与细胞的黏附情况。结果接种后5 min,三种胶原(鼠尾胶原、牛肌腱胶原、标准Ⅳ型胶原)中未贴壁的细胞数差别较大,Ⅳ型胶原中未贴壁的细胞最少;但培养至1 h,三种胶原中未贴壁的细胞数无明显差别,三者的贴壁率几乎相同;培养至第6小时三种胶原中未贴壁的细胞数仍然无明显差别,三者的贴壁率仍然基本相近。原子力显微镜可以清晰观察到胶原及胶原与细胞间黏附的微观结构。结论牛肌腱胶原和鼠尾胶原与Ⅳ型胶原对角质形成细胞的黏附作用无明显差别,原子力显微镜可以用于观察胶原及胶原与细胞间黏附的微观结构。 Objective To study the effect of different types of collagen to the adhesion of the keratinocytes, and observe the microstructure between the collagen and the cells by atomic force microscope. Methods Culture plates were covered by bovine tendon collagen, rat tail collagen and standard type Ⅳ collagen, respectively. Then the same passage of kerationcytes was seeded in one density of cells. Un-adherent cells were counted by viable count at different phases and different types of collagen. The curve of un-adherent cells at different conditions was figured, and we observed the microstrueture between the collagen and the cells by atomic forcemicroscope. Results The adhesion ability of type IV collagen to kerationcyte was higher than that of bovine tendon collagen and rat tail collagen in the first one hour, but since that, there was no significant distinction among them. Conclusion Compared with type Ⅳ collagen, collagen from bovine tendon and rat tail have similar adhesion ability to cells. The microsa'ucture between the collagens and the ceils is observed by atomic force microscope.
出处 《中华临床医师杂志(电子版)》 CAS 2013年第16期73-75,共3页 Chinese Journal of Clinicians(Electronic Edition)
基金 国家"863"高科技发展计划资助项目(2003AA205020)
关键词 显微镜检查 原子力 胶原 成纤维细胞 细胞黏附 Microscopy, atomic force Collagen Fibroblasts Cell adhesion
  • 相关文献

参考文献9

  • 1吴国选,伍津津,朱堂友,鲁元刚,毕建军.人肌腱胶原蛋白的提取及凝胶制备[J].实用医药杂志,2005,22(3):227-229. 被引量:7
  • 2周红星.组织工程中种子细胞粘附的研究进展[J].中国康复医学杂志,2004,19(8):630-633. 被引量:2
  • 3鲁元刚.胶原在皮肤组织工程中的应用[J].重庆医学,2003,32(5):609-611. 被引量:22
  • 4Bernengo J C,Herbage D,Marion C,Roux B.Intermolecular interaction studies on native and enzyme-treated acid-soluble collagen[].Biochimica et Biophysica Acta.1978
  • 5Vesenka J,Mosher C,Schaus S,Ambrosio L,Henderson E.Combining optical and atomic force microscopy for life sciences research[].Biotechniques.1995
  • 6Mikos, A.G.,Sarakinos, G.,Leite, S.M.,Vacanti, J.P.,Langer, R.Laminated three-dimensional biodegradable foams for use in tissue engineering[].Biomaterials.1993
  • 7Simon A,Cohen-Bouhacina T,Porté M C,Aimé J P,Amédée J,Bareille R,Baquey C.Characterization of dynamic cellular adhesion of osteoblasts using atomic force microscopy[].Cytometry Part A : the journal of the International Society for Analytical Cytology.2003
  • 8Mittag F,Falkenberg EM,Janczyk A,et al.Laminin-5 and type I collagen promote adhesion and osteogenic differentiation of animal serum-free expanded human mesenchymal stromal cells[].Orthop Rev(Pavia).2012
  • 9Jeong KH,Lee SH.A new technical approach to monitor the cellular physiology by atomic force microscopy[].Electrolyte Blood Press.2012

二级参考文献57

  • 1永井裕 藤本大本郎 等.胶原蛋白实验方法[M].上海:上海中医学院出版社,1992.31.
  • 2Robins SP, Duncan A, Wilson N, et al. Standardization of pyridinium crosslinks, pyridinoline and deoxypyridinoline,for use as biochemical markers of collagen degradation[J]. Clin Chem, 1996,42 : 162.
  • 3Breitbart AS, Mason JM, Urmacher C, et al. Gene-enchanced tissue engineering: application for wound healing using cultured dermalk fibroblasts tmasduced retrovirally with the PDGF-B gene[J]. Ann Plast Surg, 1999,43:632.
  • 4Machens HG, Morgan JR, Berthiaume F, et al. Genetically modified fibroblasts induce angiogensis in the 3 × 6cm rat epigastric island flap[J ]. Eur J Plast Surg, 1999,22 : 203.
  • 5Yamada KM. Cell surface interaction with extracellular materials[J]. Ann Rev Biochem, 1983,52 : 761.
  • 6Bailey Aj. The fate of collagen implants in tissue defects[J]. Wound Rep Reg,2000,8:5.
  • 7Puma Sk, Mary Babu. Collagen based dressings:a review[J]. Burns, 2000,26 : 54.
  • 8Smith DJ. Use of biobrane in wound management[J]. J Burn Care Rehabit,1995 ,16 : 317.
  • 9Lorenz CH,Petracic A, Hohl HP,et al. Early wound closure and early reconstruction. Experience with a dermal substitute in a child with 60 per cent surface area burn[J]. Burns,1997,23:505.
  • 10Soekima K, Nozaki M, Sasaki K, et al. Reconstruction of burn deformity using artificial dermis combined with thin split-skin grafting[J]. Burns, 1997,23 : 501.

共引文献27

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部