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乙型肝炎病毒对胰岛素样生长因子结合蛋白3表达的影响 被引量：1

The effect of hepatitis B virus on the expression of circulating IGF-binding protein 3
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摘要 目的探讨乙型肝炎病毒(HBV)对胰岛素样生长因子结合蛋白3(IGFBP3)表达的影响。方法逆转录-聚合酶链反应(RT-PCR)检测IGFBP3mRNA的表达,免疫印迹法检测IGFBP3的蛋白表达,酶联免疫吸附试验(ELISA)检测IGFBP3血清学含量。结果 IGFBP3mRNA和蛋白的表达在HepG2细胞中的表达水平较HepG2.2.15高;乙肝患者IGFBP3血清学水平显著降低,差异有统计学意义(P<0.05)。结论 HBV能够在体内、外抑制IGFBP3的表达。 Objective To explore the effect of hepatitis B virus（HBV） on the expression of IGF-binding protein 3（IGFBP3）. Methods mRNA and protein expression of IGFBP3 were measured by RT-PCR and Western blot. The serum levels of IGFBP3 were measured by enzyme-linked immunosorbent assay（ELISA）. Results Expression of IGFBP3 mRNA and protein were lower in HepG2.2.15 cells than in HepG2 cells. Serum IGFBP3 level reduced a lot in HBV patients （P〈0. 05）. Conclusion HI3V can downregulate the expression of IGFBP3 both in vivo and in votro.
作者 祝成亮 李艳 汪明 高国生
机构地区 武汉大学人民医院检验科 宁波市第二医院
出处 《国际检验医学杂志》 CAS 2013年第22期2947-2948,共2页 International Journal of Laboratory Medicine
基金 国家自然科学基金资助项目(81101485) 中国博士后特别资助基金资助项目(201104485) 中国博士后科学基金资助项目(20100471144) 国家临床重点专科专项经费 浙江省医药卫生一般研究计划资助项目(2011KYB107)
关键词 乙型肝炎病毒 胰岛素样生长因子结合蛋白3 酶联免疫吸附试验 表达 hepatitis B virus irculating IGF-binding protein 3 enzyme-linked immunosorbent assay expression
分类号 R512.62 [医药卫生—内科学]
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参考文献6

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二级参考文献10

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  • 10祝成亮,李艳,詹传华,郝世勇,张平安,李从荣,刘芳.乙型肝炎病毒对载脂蛋白B表达的影响及其机制探讨[J].中华微生物学和免疫学杂志,2011,31(1):30-33. 被引量：7

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国际检验医学杂志
2013年 第22期

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