孕哺期高脂膳食对成年仔鼠空间学习记忆能力的影响

Effect of maternal high fat diet during gestation and lactation period on spatial learning and memory in adult mice offspring

目的探索昆明小鼠孕哺期高脂膳食对成年期仔鼠空间学习记忆能力的影响。方法成年雌性昆明小鼠随机分为对照组和高脂组,孕哺期分别饲以标准鼠粮和含20%猪油的高脂饲料。仔鼠4周断乳,随机选取对照组10只仔鼠为对照持续组维持原来膳食;高脂组10只仔鼠为高脂持续组维持原来饲料,10只仔鼠为高脂对照组转饲以标准饲料。16周,进行Morris水迷宫测试空间学习记忆能力。称量体重、内脏脂肪和全脑质量,检测血清瘦素水平。结果高脂持续组和高脂对照组的逃避潜伏期短于对照组(P<0.05),高脂对照组的穿越平台次数高于对照持续组(P<0.05);高脂持续组和高脂对照组的体重、内脏脂肪和全脑质量均大于对照组(P<0.05);高脂持续组血清瘦素水平高于对照持续组(P<0.05)。结论孕哺期高脂膳食可提高昆明小鼠子代成年期空间学习记忆能力。

Objective To investigate the effect of perinatal high fat diet on spatial learning and memory in adult Kunming （KM） mice offspring. Methods The adult female KM mice were randomly divided into control and high fat group, fed with lab chow and high fat diet （containing 20% of lard ） during gestation and lactation periods. After weaning at 4 weeks old, 10 offspring mice randomly selected from control group, as control continuing （CC） group, were fed with lab chow. 10 mice, as high fat continuing （FF） group, from high fat group were fed with high ：[at diet. 10 mice, as transforming high fat （FC） group, were fed with lab chow. Then, Morris water maze task was applied to evaluate spatial learning and memory in the pups. The body weight and the mass of visceral fat and brain were weighted, and the level of erum leptin was measured through ELISA. Results Latency of FF and FC groups were shorter than CC group （P 〈0.05）, and the crossing times of FC group was more than CC group. The weights, the mass of visceral fat and brain of FF and FC groups were heavier than those of control group （P 〈0.05）. The level of serum leptin of FF group was higher than CC group （P 〈 0.05）. Conclusion Maternal high fat diet during pregnancy and lactation may contribute to better spatial learning and memory ability in adult mice offspring.