葡萄籽粉对高脂饲料大鼠血脂代谢和肝脏保护作用研究

Research of grape seed powder on serum lipids and hepatic protection effects in high-fat diet rats

目的研究葡萄籽粉对高脂饲料大鼠血脂代谢的影响和对保护肝脏作用。方法将Wistar大鼠随机分5组,除对照组外,其余4组给予高脂饲料。对照组和高脂组灌胃生理盐水;低、中和高剂量葡萄籽粉组分别按照标准人剂量20 g/60 kg BW的1倍、5倍和10倍的葡萄籽粉灌胃大鼠,每天灌胃一次,喂养28 d后,观察大鼠血脂和肝脏的病理变化。结果与对照组相比,高脂模型大鼠血清中总胆固醇(TC)、甘油三酯(TG)和低密度脂蛋白胆固醇(LDL-C)均有明显升高(P<0.05),血清中高密度脂蛋白胆固醇(HDL-C)降低,差异显著(P<0.05)。葡萄籽粉能显著降低高脂饲料大鼠血清TC、TG、LDL-C(P<0.05,P<0.01),增加HDL-C含量(P<0.05)。肝脏组织光镜结构表明,葡萄籽粉组肝细胞脂肪变性明显低于高脂模型组。结论葡萄籽粉具有降低血脂,预防脂肪肝的作用。

Objective To research of grape protectioneffects in high-fat diet rats. Methods seed powder on serum lipids and hepatic The rats were divided into five groups, every group wastaken high-fat diet except control group, the physiological saline was administered to the control group and the high-fat diet groups. The doses of grape seed power in low, middle and high groups were determined based on the 1 × , 5 × , and 10×~ standard human doses （20 mg/60 kg BW）, respectively. The rats were orally given test sample respectively for 28 days, once a day. Observed the changes of erum lipids and live pathology. Results Compared to the control group, the serum levels of total cholesterol （TC）, triglyceride （TG） and low density lipoprotein cholesterol （LDL-C） were increased significantly （P 〈 0.05 ） and HDL-C markedly decreased （P 〈 0.05） in high-fat diet group. The histological data with light microscopy demonstrated that the fatty degeneration in liver was significantly attenuated in grape seed powder rats as compared to high-fat diet group. Conclusion The grape seed powder can reduce the level of serum lipids and prevent the occurrence of fatty liver.