翘芩清肺剂对哮喘大鼠Th1/Th2细胞因子失衡的影响

Effects of Qiaoqinqingfei Ji on Th1/Th2 Imbalance in Rat Asthma Model

目的观察翘芩清肺剂对哮喘模型大鼠Th1/Th2失衡的免疫调节作用及机制。方法以10%卵蛋白(OVA)在实验第1天、第8天对大鼠进行腹腔注射。第15天后用1%OVA雾化吸入,每天1次,连续2周;同时,分别用生理盐水、地塞米松、氨茶碱及翘芩清肺剂干预致哮喘模型大鼠,每天1次,连续4周,观察各组大鼠哮喘潜伏期、喘息程度、肺组织、支气管病理学变化;测定肺泡灌洗液(BALF)中IL-4、IL-5、IFN-γ水平变化,并与阳性药地塞米松及氨茶碱组对照。结果翘芩清肺剂能有效延长OVA致哮喘大鼠模型的潜伏期、减轻喘息程度、改善升高的IL-4水平,降低病鼠的IL-5、INF-γ水平及IL-4/IFN-γ的比值,结果均有显著差异(P<0.05或P<0.01)。病理组织学检查模型组大鼠支气管黏膜上皮细胞及杯状细胞增生,肺泡内有分泌物及炎性细胞浸润,管腔狭窄;各用药物组病理改变均显著减轻。结论翘芩清肺剂可能通过调节哮喘时失衡的Th1/Th2水平,有效改善哮喘状态,减轻哮喘发作。

OBJECTIVE To investigate the effect of Qiaoqinqingfei Ji on immune regulation Th1/Th2 imbalance and its mechanism in the rat asthmatic models. METHODS Asthmatic model radts were established by introperitoneal injection of 10% OVA on the first day and the eighth day. Fifteen days after, gave atomization inhale 1% OVA, once a day for two weeks. At the same time, normal saline, dexamethasone, aminophylline and Qiaoqinqingfei Ji were used for intervention treatment respectively to asthma model rats, once a day, last for four weeks. The effect of medicine on the asthma degree, asthma latency period and the pathological changes of bronchial and lung tissue, the contents of IFN-γ, IL-4, lL-5 and the IFN-γ/IL-4 ratio inbronchus alveolus lavage fluid of asthma model were observed. And compared with dexamethason group and aminophylline group. RESULTS Qiaoqingqifei Ji obviously improved the asthma degree,, extended asthma latency period, decreased the content of IL-4, IL-5（P〈0.05 or P〈0.01）, increased the content of IFN-γ and the ratio of IFN-γ/IL-4. Pathological results revealed that the bronchial mucosa epithelial cells and goblet cells hyperplasia, alveolar contains secretions and inflammatory cells infiltration, luminal stenosis in the model group. And these changes were released obviously in the medicine group. CONCLUSION Qiaoqingqifei Ji can effectively adjust Th1/Th2 imbalance in asthmatic rats, improve asthma status and reduce asthma attacks.