HPLC-ELSD测定地黄寡糖中地黄苷A含量

Determination of Rehmannioside A in Rehmannia Glutinosa Oligosaccharide by HPLC-ELSD

目的 建立测定地黄寡糖中地黄苷A的高效液相色谱法。方法 采用Agilent TC C18色谱柱（4.6mm×250mm,5μm）,柱温35℃,流动相：甲醇-水（10∶90）,流速1.0mL·min1;ELSD参数：漂移管温度110℃,载气流量3.2L·min1。结果 地黄苷A在0.2~1.2mg·mL1（r=0.9993）内线性关系良好,平均回收率为98.8%（RSD=1.6%）。结论 该方法准确、简便、专属性好,可用于地黄寡糖中地黄苷A成分的含量测定,为地黄寡糖的质量控制提供了依据。

OBJECTIVE To establish an HPLC method to determine the content of rehmannioside A in Rehmannia glutinosa oligosaccharide. METHODS The HPLC-ELSD was used with Agilent TC C18 column （4.6 mm×250 mm, 5 μm） at 35 ℃. The mobile phase was methanol and water（10 ： 90） with a flow rate 1.0 mL·min-1; The temperature of the drift tube in ELSD was 110 ℃ and the flow rate of dry air was 3.2 L·min-1. RESULTS The rehmannioside A had a good linear relationship between 0.2-1.2 mg＇mL-1. CONCLUSION The method is accurate, simple with good specificity, and it could be used to determine the content of rehmannioside A in Rehmannia glutinosa oligosaccharide, which may be providing a evidence for the quality control ofRehmannia glutinosa oligosaccharide.