巨噬细胞炎症蛋白-1α在慢性阻塞性肺疾病急性加重期患者血清中的变化

The changes of serum level of MIP-1α in patients with acute exacerbation of chronic obstructive pulmonary disease

目的:通过检测慢性阻塞性肺疾病急性加重期(AECOPD)患者治疗前后血清中巨噬细胞炎症蛋白1-α(MIP-1α)水平,探讨MIP-1α在COPD中的变化及意义。方法:COPD急性加重期患者47例(COPD组),按肺功能分为轻、中、重3组。同时选取健康自愿者(正常对照组)30例,采用双抗体夹心ELISA法对COPD各组入院时和经过治疗病情缓解后及对照组患者血清中MIP-1α进行定量测定。COPD组同步行肺功能检测,并将各组患者所测得的结果与正常对照组对比分析。结果:COPD各组治疗前血清中MIP-1α较正常组明显升高(P<0.05),治疗后水平下降,但仍高于正常对照组(P<0.05)。COPD各组治疗前后血清MIP-1α水平随分级增加而增加,轻、中、重度组间差异有统计学意义(P<0.05)。结论:MIP-1α参与了COPD的发病过程,持续异常升高的MIP-1α可能是造成COPD患者气道慢性炎症的不可逆转原因之一,且其升高程度与病情严重程度有关。

Objective To investigate the changes of serum level of macrophage inflammatory protein-1α （MIP-1α） in patients with acute exacerbation of chronic obstructive puhnonary disease （AECOPD） after treatment. Methods 47 patients with AECOPD （AECOPD group） were divided into 3 sub-groups according to the lung function ： mild group, moderate group and severe group. Another 30 healthy controls were included as control group. Serum levels of MIP-1α in AECOPD group and control group were measured by enzyme-linked immunosorhent assay （ELISA） ,and meanwhile lung function test was administered. Results Serum level of MIP-1α in AECOPD group before the treatment was significantly higher than that in control group ; and was decreased after the treatment, which was still higher than that in control group （P 〈 0.05）. There were significant differences of serum level of MIP-1α among the 3 sub-groups of AECOPD patients, and that was increased with the increasing of the grade of AECOPD （P 〈 0.05）. Conclusion MIP-1α may contribute to the development of COPD, and continuous high level of MIP-1α may be one of the important factors causing chronic airway inflammation which cannot be reversed. Level of M1P-1α was related to the severity of COPD.